胃癌组织YAP1、E-cadherin、N-cadherin表达及临床意义
| 作者: |
1肖磊,
1黄昌浩,
1袁伟杰,
1陈晋湘,
2骆晴晴,
1陈子华
1 中南大学湘雅医院胃肠外科，湖南 长沙 410008 2 中南大学湘雅医院 肿瘤科，湖南 长沙 410008 |
| 通讯: |
陈子华
Email: zihuac@126.com |
| DOI: | 10.3978/.2018.04.009 |
| 基金: | 湖南省自然科学基金资助项目（2015JJ4049）。 |
摘要
目的：探讨YAP1、E-cadherin、N-cadherin在胃癌组织中表达及意义。
方法：用免疫组化法检测胃癌组织芯片中135例患者癌组织和癌旁组织中YAP1、E-cadherin、N-cadherin表达，分析YAP1表达与E-cadherin、N-cadherin表达的关系，以及与胃癌患者临床病理因素以及预后的关系。
结果：与癌旁组织比较，胃癌组织中YAP1表达明显升高，E-cadherin表达明显降低（均P<0.05），N-cadherin表达无明显差异（P>0.05）；在胃癌组织中，YAP1表达与E-cadherin表达呈负相关（r=-0.273，P=0.032），与N-cadherin表达呈正相关（r=0.304，P=0.019）。YAP1在胃癌组织中的表达与胃癌患者淋巴转移、脉管侵犯有关（均P<0.05），且YAP1阳性表达患者总生存率明显低于阴性表达患者（χ2=4.354，P=0.037）。
结论：YPA1与E-cadherin在胃癌组织中表达有明显变化，YAP1高表达与胃癌患者不良临床病理特征及预后密切相关。
关键词：
胃肿瘤；YES相关蛋白；上皮-间质转化；预后
方法：用免疫组化法检测胃癌组织芯片中135例患者癌组织和癌旁组织中YAP1、E-cadherin、N-cadherin表达，分析YAP1表达与E-cadherin、N-cadherin表达的关系，以及与胃癌患者临床病理因素以及预后的关系。
结果：与癌旁组织比较，胃癌组织中YAP1表达明显升高，E-cadherin表达明显降低（均P<0.05），N-cadherin表达无明显差异（P>0.05）；在胃癌组织中，YAP1表达与E-cadherin表达呈负相关（r=-0.273，P=0.032），与N-cadherin表达呈正相关（r=0.304，P=0.019）。YAP1在胃癌组织中的表达与胃癌患者淋巴转移、脉管侵犯有关（均P<0.05），且YAP1阳性表达患者总生存率明显低于阴性表达患者（χ2=4.354，P=0.037）。
结论：YPA1与E-cadherin在胃癌组织中表达有明显变化，YAP1高表达与胃癌患者不良临床病理特征及预后密切相关。
Expressions of YAP1, E-cadherin and N-cadherin in gastric cancer tissue and their clinical significance
CorrespondingAuthor:CHEN Zihua Email: zihuac@126.com
Abstract
Objective: To investigate the expressions of YAP1, E-cadherin and N-cadherin in gastric cancer tissue and their significance.
Methods: The expressions of YAP1, N-cadherin and E-cadherin proteins in tissue microarray containing 135 samples of gastric cancer tissue and adjacent tissue were determined by immunohistochemical staining. The relations of YAP1 expression with E-cadherin and N-cadherin expressions as well as with clinicopathologic characteristics and prognosis of the patients were analyzed.
Results: In gastric cancer tissue compared with adjacent non-tumor tissue, the YAP1 expression was increased and E-cadherin expression was decreased significantly (both P<0.05), but the N-cadherin showed no significant difference (P>0.05). There was a negative correlation between YAP1 expression and E-cadherin expression (r=–0.273, P=0.032), and a positively correlation between YAP1 expression and N-cadherin (r=0.304, P=0.019) in gastric cancer tissue. The expression of YAP1 in gastric cancer was correlated with lymphatic metastasis and vascular invasion (P<0.05), and the overall survival rate in patients with YAP1 positive expression was significantly lower than that in those with YAP1 negative expression (χ2=4.354, P=0.037).
Conclusion: The expressions of YPA1 and E-cadherin are significantly changed in gastric cancer tissue, and high YAP1 expression is closely related to the unfavorable clinicopathologic features and poor prognosis in the gastric cancer patients.
Keywords：
Stomach Neoplasms; Yes-Associated Proteins; Epithelial-Mesenchymal Transition; Prognosis
Methods: The expressions of YAP1, N-cadherin and E-cadherin proteins in tissue microarray containing 135 samples of gastric cancer tissue and adjacent tissue were determined by immunohistochemical staining. The relations of YAP1 expression with E-cadherin and N-cadherin expressions as well as with clinicopathologic characteristics and prognosis of the patients were analyzed.
Results: In gastric cancer tissue compared with adjacent non-tumor tissue, the YAP1 expression was increased and E-cadherin expression was decreased significantly (both P<0.05), but the N-cadherin showed no significant difference (P>0.05). There was a negative correlation between YAP1 expression and E-cadherin expression (r=–0.273, P=0.032), and a positively correlation between YAP1 expression and N-cadherin (r=0.304, P=0.019) in gastric cancer tissue. The expression of YAP1 in gastric cancer was correlated with lymphatic metastasis and vascular invasion (P<0.05), and the overall survival rate in patients with YAP1 positive expression was significantly lower than that in those with YAP1 negative expression (χ2=4.354, P=0.037).
Conclusion: The expressions of YPA1 and E-cadherin are significantly changed in gastric cancer tissue, and high YAP1 expression is closely related to the unfavorable clinicopathologic features and poor prognosis in the gastric cancer patients.