长链非编码RNA TUG1和UCA1在结肠癌组织中的表达 及临床意义
| 作者: |
1姚凯,
1朱光辉,
1单远洲
1 上海交通大学附属第六人民医院南院 普通外科，上海 201499 |
| 通讯: |
单远洲
Email: shanyuanzhou2014@sina.com |
| DOI: | 10.3978/.2018.04.010 |
摘要
目的：探讨结肠癌组织中长链非编码RNA TUG1和UCA1的表达及临床意义。
方法：收集2010年1月—2014年6月外科行手术治疗的185例结肠癌患者的肿瘤及对应癌旁非肿瘤组织冻存标本，采用原位杂交与qRT-PCR法检测TUG1和UCA1的表达，分析两者表达与患者临床病理特征及预后的关系。
结果：UCA1和TUG1在结肠癌组织中的阳性表达率与相对表达量均明显癌旁高于非肿瘤组织（均P<0.05）。UCA1和TUG1表达均与肿瘤大小、淋巴结转移、分化程度、TNM分期等临床特征均有明显有关（均P<0.05）。生存分析显示，UCA1与TUG1高表达患者总体生存率明显相应的低于UCA1低表达患者（χ2=5.491，P=0.019；χ2=4.345，P=0.037）。UCA1与TUG1的表达均是结肠癌患者预后的独立危险因素（均P<0.05）。
结论：UCA1和TUG1在结肠癌肿瘤组织中表达上调，两者的高表达与结肠癌的进展及患者的不良预后密切相关。
关键词：
结肠肿瘤；RNA，长链非编码；预后
方法：收集2010年1月—2014年6月外科行手术治疗的185例结肠癌患者的肿瘤及对应癌旁非肿瘤组织冻存标本，采用原位杂交与qRT-PCR法检测TUG1和UCA1的表达，分析两者表达与患者临床病理特征及预后的关系。
结果：UCA1和TUG1在结肠癌组织中的阳性表达率与相对表达量均明显癌旁高于非肿瘤组织（均P<0.05）。UCA1和TUG1表达均与肿瘤大小、淋巴结转移、分化程度、TNM分期等临床特征均有明显有关（均P<0.05）。生存分析显示，UCA1与TUG1高表达患者总体生存率明显相应的低于UCA1低表达患者（χ2=5.491，P=0.019；χ2=4.345，P=0.037）。UCA1与TUG1的表达均是结肠癌患者预后的独立危险因素（均P<0.05）。
结论：UCA1和TUG1在结肠癌肿瘤组织中表达上调，两者的高表达与结肠癌的进展及患者的不良预后密切相关。
Expressions of long noncoding RNA TUG1 and UCA1 in colon cancer tissue and their clinical significance
CorrespondingAuthor:SHAN Yuanzhou Email: shanyuanzhou2014@sina.com
Abstract
Objective: To investigate the expression of the long noncoding RNA TUG1 and UCA1 in colon cancer tissue and their clinical significance.
Methods: The frozen specimens of cancer tissues along with their adjacent tissues from 185 colon cancer patients undergoing surgical resection from January 2010 to June 2014 were collected. The expressions of TUG1 and UCA1 were determined by in situ hybridization and qRT-PCR respectively, and the relations of their expressions with the clinicopathologic features and prognosis of the patients were analyzed.
Results: Both positive expression rates and relative expression levels of UCA1 and TUG1 in colon cancer tissue were significantly higher than those in the adjacent non-tumor tissue (all P<0.05). The expressions of UCA1 and TUG1 were significantly related to the clinicopathologic factors that included tumor size, lymph node metastasis, degree of differentiation and TNM stage (all P<0.05). Survival analysis showed that the overall survival rate in patients with high UCA1 expression or high TUG1 expression was significantly lower than that in patients with low UCA1 expression or low TUG1 expression (χ2=5.491, P=0.019; χ2=4.345, P=0.037). Both UCA1 and TUG1 expressions were independent risk factors for prognosis of the colon cancer patients (both P<0.05).
Conclusion: The expressions of UCA1 and TUG1 are up-regulated in colon cancer tissue, and their high expressions are closely associated with the progression of colon cancer and poor prognosis of the patients.
Keywords：
Colonic Neoplasms; RNA
Long Noncoding; Prognosis
Methods: The frozen specimens of cancer tissues along with their adjacent tissues from 185 colon cancer patients undergoing surgical resection from January 2010 to June 2014 were collected. The expressions of TUG1 and UCA1 were determined by in situ hybridization and qRT-PCR respectively, and the relations of their expressions with the clinicopathologic features and prognosis of the patients were analyzed.
Results: Both positive expression rates and relative expression levels of UCA1 and TUG1 in colon cancer tissue were significantly higher than those in the adjacent non-tumor tissue (all P<0.05). The expressions of UCA1 and TUG1 were significantly related to the clinicopathologic factors that included tumor size, lymph node metastasis, degree of differentiation and TNM stage (all P<0.05). Survival analysis showed that the overall survival rate in patients with high UCA1 expression or high TUG1 expression was significantly lower than that in patients with low UCA1 expression or low TUG1 expression (χ2=5.491, P=0.019; χ2=4.345, P=0.037). Both UCA1 and TUG1 expressions were independent risk factors for prognosis of the colon cancer patients (both P<0.05).
Conclusion: The expressions of UCA1 and TUG1 are up-regulated in colon cancer tissue, and their high expressions are closely associated with the progression of colon cancer and poor prognosis of the patients.