基础研究(Basic Research)

硫利达嗪对胃癌细胞生长的抑制作用与机制研究

Published at: 2015年第24卷第10期

王磊 1 , 汪宏 2
1 安徽医科大学,安徽 合肥 230022
2 安徽医科大学第三附属医院/ 安徽省合肥市第一人民医院 微创外科,安徽 合肥 230061
通讯作者 宏 汪 Email: whsurgery1958@163.com
DOI: 10.3978/j.issn.1005-6947.10.3978/j.issn.1005-6947.2015.10.011
基金:

摘要

目的:探讨硫利达嗪对胃癌细胞体外生长的抑制作用及其机制。方法:不同浓度的硫利达嗪作用于胃癌SGC-7901 细胞24 h 后,分别用MTT 法与流式细胞仪检测胃癌细胞的增殖与凋亡情况,以及用Western blot 检测凋亡相关蛋白bax、bcl-2、caspase-3 的表达情况。结果:硫利达嗪作用后,SGC-7901 细胞的增殖明显抑制,凋亡率明显增加,抗凋亡蛋白bcl-2 表达下调、促凋亡蛋白bax 表达上调、caspcase-3 蛋白表达上调,且均呈浓度依赖性(均P<0.05)。结论:硫利达嗪对人胃癌细胞体外的生长有明显抑制作用,该作用可能与其并活化caspase-3 依赖的凋亡途径有关。


Inhibitory effect of thioridazine on growth of gastric cancer cells and its mechanism

Abstract

Objective: To investigate the inhibitory effect of thioridazine on growth of gastric cancer cells in vitro and the mechanism. Methods: Gastric cancer SGC-7901 cells were exposed to different concentrations of thioridazine for 24 hours, and then the proliferation and apoptosis of the gastric cells were detected by MTT assay and flow cytometry, and the expressions of apoptosis-related proteins that included bax, bcl-2 and caspase-3 were measured by Western blot analysis, respectively. Results: In SGC-7901 cells after exposure to thioridazine, the proliferation was decreased, apoptosis was increased significantly, the expression of antiapoptotic protein bcl-2 was down-regulated, and proapoptotic protein bax as well as caspase-3 was up-regulated significantly, and all effects showed a concentration-dependent manner (all P<0.05). Conclusion: Thioridazine has evident inhibitory effect on human gastric cancer in vitro, which may possibly be associated with its activating the caspase-3-dependent apoptotic pathway.


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引用

引用本文: 磊 王, 宏 汪. 硫利达嗪对胃癌细胞生长的抑制作用与机制研究[J]. 中国普通外科杂志, 2015, 24(10): 1401-1405.
Cite this article as: WANG Lei, WANG Hong . Inhibitory effect of thioridazine on growth of gastric cancer cells and its mechanism[J]. Chin J Gen Surg, 2015, 24(10): 1401-1405.