基础研究(Basic Research)

缺血预处理对大鼠缺血再灌注肝组织NF-κB 表达、炎症及氧化应激反应的影响

Published at: 2015年第24卷第1期

陶立德 1 , 薛同敏 1 , 张杰 1 , 张培建 1
1 扬州大学附属第二临床医院 普通外科研究室,江苏 扬州 215000
通讯作者 培建 张 Email: yzu.edu.pjz@163.com
DOI: 10.3978/j.issn.1005-6947.10.3978/j.issn.1005-6947.2015.01.013
基金:
江苏省扬州市自然科学基金面上资助项目 YZ2014064

摘要

目的:探讨缺血预处理(IP)减轻大鼠肝缺血再灌注(I/R)损伤的作用及机制。 方法:将15 只雄性SD 大鼠随机均分为假手术组、I/R 组、IP+I/R 组,采用Pringle 法制作肝I/R 模型 (缺血30 min+ 再灌注3 h),IP 采用I/R 前肝缺血10 min+ 再灌注10 min 诱导。各组大鼠于再灌注3 h 后处死取材,行肝组织病理学、血请谷草转氨酶(AST)、谷丙转氨酶(ALT)检测,同时检测肝组织 NF-κB 蛋白的表达,以及炎性细胞因子IL-1β、TNF-α 与氧化应激指标丙二醛(MDA)、髓过氧化 物酶(MPO)水平。 结果:除假手术组外,I/R 组与IP+I/R 组大鼠肝组织均出现肝损伤是病理学改变,IP+I/R 组的损伤程 度明显轻于I/R 组;与假手术组比较,I/R 组与IP+I/R 组大鼠血清AST、ALT 水平明显升高,肝组织 NF-κB 蛋白表达、IL-1β 与TNF-α 水平、MDA 与MPO 浓度均明显升高(均P<0.05),但IP+I/R 组 的各指标的升高幅度均明显小于I/R 组(均P<0.05)。 结论:IP 减轻大鼠肝I/R 损伤的作用与抑制NF-κB 活性,从而减轻炎症与氧化应激反应有关。


Influence of ischemic preconditioning on NF- B expression, and inflammatory and oxidative stress responses in rat liver tissue following ischemia-reperfusion

Abstract

Objective: To investigate the alleviation effect of ischemic preconditioning (IP) on hepatic ischemia-reperfusion (I/R) injury in rats and the mechanism. Methods: Fifteen male SD rats were equally randomized into sham operation group, I/R group and IP plus I/ R group, respectively. I/R injury model was created by Pringle maneuver (30-min hepatic ischemia followed by 3-h reperfusion), and IP was induced by 10-min hepatic ischemia followed by 10-min reperfusion prior to I/R. Rats in each group were sacrificed and samples were collected after 3-h reperfusion, liver specimen pathological examination and measurement of serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were performed, and meanwhile, the NF-κB protein expression, and the levels of inflammatory cytokines (IL-1β and TNF-α) as well as oxidative stress indexes that included malondialdehyde (MDA) and myeloperoxidase (MPO) in the liver tissues were determined. Results: Except in sham operation group, the liver tissues from either I/R group or IP plus I/R group showed pathological changes of liver injury, but the injury was milder in IP plus I/R group than that in I/R group. In both I/R group and IP plus I/R group compared with sham operation group, the serum AST and ALT levels, and liver tissue levels of NF-κB protein expression, IL-1β, TNF-α, MDA and MPO were all significantly increased (all P<0.05), but the increasing amplitudes of all these parameters in IP plus I/R group were significantly less than those in I/R group (all P<0.05). Conclusion: IP lessens hepatic I/R through inhibiting NF-κB activity, and thereby reduces inflammatory and oxidative stress responses.


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引用

引用本文: 立德 陶, 同敏 薛, 杰 张, 培建 张. 缺血预处理对大鼠缺血再灌注肝组织NF-κB 表达、炎症及氧化应激反应的影响[J]. 中国普通外科杂志, 2015, 24(1): 70–74.
Cite this article as: TAO Lide, XUE Tongmin, ZHANG Jie, ZHANG Peijian . Influence of ischemic preconditioning on NF- B expression, and inflammatory and oxidative stress responses in rat liver tissue following ischemia-reperfusion [J]. Chin J Gen Surg, 2015, 24(1): 70–74.