基础研究(Basic Research)

HMGB1 促进血管平滑肌细胞增殖与迁移的机制研究

Published at: 2015年第24卷第12期

徐韶飞 1 , 聂晚频 1 , 姚凯 1 , 王征 1
1 中南大学湘雅三医院 血管外科,湖南 长沙 410013
通讯作者 晚频 聂 Email: niewanpin@medmail.com.cn
DOI: 10.3978/j.issn.1005-6947.10.3978/j.issn.1005-6947.2015.12.013
基金:

摘要

目的:探讨高迁移率蛋白B1(HMGB1)促进血管平滑肌细胞(VSMC)增殖与迁移的机制。 方法:检测人主动脉血管平滑肌细胞(HA-VSMC)与HMGB1 孵育后增殖与迁移的活性、晚期糖基化 终产物受体(RAGE)与P38MAPK 表达改变,以及RAGE 抗体、P38MAPK 抑制剂SB203580 预处理 的影响。 结果:HMGB1 孵育后,HA-VSMC 增殖与迁移活性、RAGE 和P38MAPK 的表达均明显增加( 均 P<0.05),且均呈一定的浓度依赖性。用RAGE 抗体和SB203580 预处理后,HMGB1 促进HA-VSMC 增殖及迁移的作用均被明显抑制(均P<0.05),RAGE 抗体预处理后,HMGB1 对P38MAPK 表达的诱 导作用明显抑制(P<0.05)。 结论:HMGB1 可能通过与细胞表面RAGE 受体结合,激活P38MAPK 表达进而促进VSMC 的增殖及迁移。


Mechanism for HMGB1 in promoting migration and proliferation of vascular smooth muscle cells

Abstract

Objective: To investigate the mechanism of high mobility of protein B1 (HMGB1) in promoting migration and proliferation of vascular smooth muscle cells (VSMCs). Methods: The changes in migration and proliferation ability as well as the expressions of receptor for advanced glycation end-products (RAGE) and P38MAPK were measured in human aortic VSMCs (HA-VSMCs) after exposure to HMGB1. Further, the influence of RAGE antibody or P38MAPK inhibitor SB203580 pretreatment was observed. Results: After exposure to HMGB1, the activity of the cell proliferation and migration, as well as the expression of RAGE and P38MAPK were increased significantly (all P<0.05), and all presented in a concentration-dependent manner. The promoting effects of HMGB1 on migration and proliferation ability were significantly inhibited by either RAGE antibody or SB203580 pretreatment (all P<0.05), and HMGB1-induced P38MAPK expression was significantly inhibited by RAGE antibody pretreatment (P<0.05). Conclusion: HMGB1 can probably promote the migration and proliferation of VSMCs through its binding to cell surface RAGE and then activating P38MAPK expression.


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引用

引用本文: 韶飞 徐, 晚频 聂, 凯 姚, 征 王. HMGB1 促进血管平滑肌细胞增殖与迁移的机制研究[J]. 中国普通外科杂志, 2015, 24(12): 1703-1708.
Cite this article as: XU Shaofei, NIE Wanpin, YAO Kai, WANG Zheng . Mechanism for HMGB1 in promoting migration and proliferation of vascular smooth muscle cells[J]. Chin J Gen Surg, 2015, 24(12): 1703-1708.