基础研究(Basic Research)

柠檬酸钠促胃癌MGC-803细胞凋亡的作用及机制研究

Published at: 2016年第25卷第4期

郭兴裕 1 , 王婷安 1 , 张晓东 1 , 冼书林 1 , 陆云飞 1
1 广西医科大学第一附属医院 胃肠腺体外科,广西 南宁 530021
通讯作者 云飞 陆 Email: doctorlife@126.com
DOI: 10.3978/j.issn.1005-6947.10.3978/j.issn.1005-6947.2016.04.012
基金:
国家自然科学基金资助项目 81260366

摘要

目的:探讨柠檬酸钠(SCT)促进胃癌MGC-803细胞凋亡的作用及机制。方法:胃癌MGC-803细胞分别经SCT(5、10、20 mmol/L)和5-FU(0.5 mmol/L)作用,以未处理的MGC-803为阴性对照,用流式细胞仪检测细胞周期分布及细胞凋亡率;比色法检测细胞内乳酸含量、磷酸果糖激酶1(PFK-1)活性及三磷酸腺苷(ATP)水平;Western blot检测细胞中Bcl-2、Bax、caspase-3及Cyt-c蛋白的相对表达量。结果:与阴性对照细胞比较,SCT处理的MGC-803细胞G2/M期阻滞与细胞凋亡明显增加;细胞内乳酸含量、PFK-1的活性和ATP水平均明显降低;细胞内Bcl-2的表达明显降低,而Bax、caspase-3和Cyt-c表达明显升高(均P<0.05)。5-FU对MGC-803细胞乳酸含量、PFK-1的活性无明显影响(均P>0.05),但其他作用与SCT相似。结论:SCT可促进MGC-803细胞凋亡,其作用可能与其抑制PFK-1的活性,降低糖酵解效率,并与线粒体凋亡通路的激活有关。


Promoting effect of sodium citrate on apoptosis in gastric cancer cells and its mechanism

Abstract

Objective: To investigate the effect of sodium citrate (SCT) on promoting apoptosis in gastric cancer cells and its mechanism. Methods: Gastric cancer MGC-803 cells were exposed to SCT (5, 10, 20 mmol/L) or 5-FU (0.5 mmol/L) respectively, using untreated MGC-803 cells as negative control. Then, the cell cycle distribution and apoptotic rate were analyzed by flow cytometry, the intracellular lactate content, phosphofructokinase-1 (PFK-1) activity and adenosine triphosphate (ATP) level were examined by colorimetric assay, and the expression of Bcl-2, Bax, caspase-3 and Cyt-c in MGC-803 cells were determined by Western blot analysis. Results: In SCT treated MGC-803 cells compared with negative control cells, the apoptosis and G2/M arrest were significantly increased, the intracellular lactate content, PFK-1 activity and ATP level were significantly reduced and Bcl-2 expression was significantly down-regulated, while the expressions of Bax, caspase-3 and Cyt-c were significantly up-regulated (all P<0.05). 5-FU exerted no significant effect on lactate content and PFK-1 activity in MGC-803 cells (all P>0.05), but all other effects were similar to those of SCT. Conclusion: SCT can promote apoptosis in MGC-803 cells, and the mechanism may be associated with its reducing glycolysis via inhibiting PFK-1 activity and activating mitochondria-dependent apoptotic pathway.


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引用

引用本文: 兴裕 郭, 婷安 王, 晓东 张, 书林 冼, 云飞 陆. 柠檬酸钠促胃癌MGC-803细胞凋亡的作用及机制研究[J]. 中国普通外科杂志, 2016, 25(4): 535-540.
Cite this article as: GUO Xingyu, WANG Ting’an, ZHANG Xiaodong, XIAN Shulin, LU Yunfei . Promoting effect of sodium citrate on apoptosis in gastric cancer cells and its mechanism [J]. Chin J Gen Surg, 2016, 25(4): 535-540.