目的：探讨Notch1、Notch3及Hes1在胃肠道间质瘤（GIST）中的表达及临床意义。方法：收集48例手术切除的原发性GIST组织标本以及48例GIST瘤旁胃肠组织标本，用免疫组化法检测Notch1、Notch3及下游靶基因Hes1蛋白的表达，并分析各蛋白表达与GIST患者临床病理因素的关系。结果：与瘤旁胃肠组织比较，GIST组织中Notch1（58.33% vs. 29.17%）、Notch3（97.97% vs. 35.47%）、Hes1（35.47% vs. 10.42%）蛋白的阳性表达率均明显升高（均P<0.05）。将GIST患者按性别、年龄、肿瘤部位、肿瘤大小及NIH危险分级分组比较各蛋白的表达阳性率，结果显示，Notch1蛋白表达与GIST的NIH危险分级有关（χ2=16.53，P=0.001）；Notch3蛋白的表达与以上各因素级均无关（均P>0.05）；Hes1蛋白表达与GIST的大小有关（χ2=7.78，P=0.02）。结论：Notch信号通路相关蛋白在GIST组织中表达升高，且可能在GIST的发生、发展中起重要作用。
Expressions of Notch signaling pathway related proteins in gastrointestinal stromal tumors and their clinical significance
Objective: To investigate expressions of Notch1, Notch3 and Hes1in gastrointestinal stromal tumors(GIST) and their clinical significance. Methods: The tissue specimens of 48 cases of resected GIST and tissue specimens of gastrointestinal tissue adjacent to 48 cases of GIST were collected. The protein expressions of Notch1 and Notch3 along with the down-steam target gene Hes1 were determined by immunohistochemical staining, and the relations of each protein expression with the clinicopathologic factors of GIST patients were also analyzed. Results: IIn GIST tissue compared with its adjacent gastrointestinal tissue, the positive expression rate of either Notch1 (58.33% vs. 29.17%), Notch3 (97.97% vs. 35.47%) or Hes1 (35.47% vs. 10.42%) protein was significantly increased (all P<0.05). After comparison of the positive expression rate of each protein between or among groups of GIST patients stratified by gender, age, tumor site, tumor size and NIH risk classification, the results showed that Notch1 expression was significantly associated with NIHrisk classification (χ2=16.53, P=0.001), Notch3 expression was irrelevant to any of the above factors (all P>0.05) and Hes1 expression was significantly related to tumor size (χ2=7.78, P=0.02). Conclusion: The expressions of Notch signaling pathway related proteins are increased in GIST tissue, which may play an important role in the occurrence and development of GIST.