基础研究(Basic Research)

膜联蛋白A2、上皮细胞钙黏蛋白及波形蛋白在肝内胆管结石相关肝内胆管癌中的表达及意义

Published at: 2017年第26卷第2期

柳志 1 , 陈勇治 1 , 舒健 1 , 符洋 1 , 王俊 1 , 吕品 1 , 张红辉 1 , 周正 1
1 湖南师范大学第一附属医院/湖南省人民医院 肝胆外科, 湖南 长沙 410005
通讯作者 俊 王 Email: 13973120355@139.com
DOI: 10.3978/j.issn.1005-6947.10.3978/j.issn.1005-6947.2017.02.010
基金:
湖南省教育厅高校科研计划资助项目 15C0836
湖南省长沙市科学技术局科技计划资助项目 K1203042-31
2016年湖南省研究生科研创新资助项目 CX2016B224

摘要

目的:探讨膜联蛋白A2(annexin A2)与上皮-间质转化(EMT)标志物上皮细胞钙黏蛋白(E-cadherin)及波形蛋白(vimentin)在肝内胆管结石相关肝内胆管癌中的表达及意义。方法:用免疫组化检测46例肝内胆管结石相关肝内胆管癌组织(肿瘤组)、50例单纯肝内胆管结石慢性炎症胆管组织(炎症组)及35例来自血管瘤旁和肝外伤的正常胆管组织(正常组)中annexin A2、E-cadherin、vimentin的表达,比较各组3种蛋白表达的差异,并分析3种蛋白的表达与肿瘤组患者临床病理特征及预后的关系。结果:annexin A2与vimentin的阳性表达率在肿瘤组、炎症组、正常组均呈依次明显降低(69.6% vs. 36.0% vs. 11.4%;54.3% vs. 28.0% vs. 8.6%,均P<0.05),而E-cadherin的阳性表达率则相反(21.7% vs. 48.0% vs. 71.4%,均P<0.05);在胆管癌组织中,annexin A2与vimentin的表达呈正相关(r=0.627,P<0.05),E-cadherin的表达与annexin A2、vimentin的表达均呈负相关(r=-0.682;r=-0.575,均P<0.05)。annexin A2、vimentin的表达与肿瘤的分化程度、淋巴结转移、TNM分期有关,E-cadherin的表达与肿瘤的分化程度、淋巴结转移有关(均P<0.05)。肝内胆管结石相关肝内胆管癌患者中,annexin A2和vimentin各自阳性者的生存率低于其各自阴性者,E-cadherin阴性者生存率低于其阳性者(均P<0.05)。结论:annexin A2/EMT通路在肝内胆管结石相关肝内胆管癌的发生和发展中可能至关重要,annexin A2、E-cadherin及vimentin的表达与肿瘤的恶性程度和患者预后密切相关。


Expressions of annexin A2, E-cadherin and vimentin in hepatolithiasis-associated intrahepatic cholangiocarcinoma and their significance

Abstract

Objective: To investigate the expressions of annexin A2 and the epithelial-mesenchymal transition (EMT) markers E-cadherin and vimentin in hepatolithiasis-associated intrahepatic cholangiocarcinoma and their significance. Methods: The expressions of annexin A2, E-cadherin and vimentin in tumor tissues from 46 patients with hepatolithiasis-associated intrahepatic cholangiocarcinoma (tumor group), bile duct tissues with chronic inflammation from 50 patients with simple hepatolithiasis (inflammation group) and normal bile duct tissues form 35 patients undergoing surgical resection for hepatic hemangioma or liver injury (normal group) were determined by immunohistochemical staining. The expressions of the three proteins among the groups were compared and their relations with clinicopathologic factors and prognosis of the patients with hepatolithiasis-associated intrahepatic cholangiocarcinoma were analyzed. Results: The positive expression rates of both annexin A2 and vimentin were presented in descending order as tumor group>inflammation group>normal group (69.6% vs. 36.0% vs. 11.4%; 54.3% vs. 28.0% vs. 8.6%, all P<0.05), while the opposite pattern was seen in that of E-cadherin (21.7% vs. 48.0% vs. 71.4%, all P<0.05). In cholangiocarcinoma tissues, there was a positive correlation between the annexin A2 and vimentin expression (r=0.627, P<0.05), and negative correlation between the E-cadherin expression and either the annexin A2 or vimentin expression (r=–0.682; r=–0.575, both P<0.05). The expressions of annexin A2 and vimentin were significantly associated with the degree of tumor differentiation, lymph node metastasis and TNM stage, and E-cadherin expression was significantly associated with the degree of tumor differentiation and lymph node metastasis (all P<0.05). Among the patients with hepatolithiasis-associated intrahepatic cholangiocarcinoma, the survival rate in cases with positive annexin A2 or vimentin expression was significantly lower than that in their corresponding negative ones, and the survival rate in cases with negative E-cadherin expression was significantly lower than that in those with its positive expression (all P<0.05). Conclusion: The annexin A2/EMT pathway may probably play an important role in the development and progression of hepatolithiasis-associated intrahepatic cholangiocarcinoma. The expressions of annexin A2, E-cadherin and vimentin are closely related to the degree of malignancy of the tumor and prognosis of the patients.


comments powered by Disqus

全文

引用

引用本文: 志 柳, 勇治 陈, 健 舒, 洋 符, 俊 王, 品 吕, 红辉 张, 正 周. 膜联蛋白A2、上皮细胞钙黏蛋白及波形蛋白在肝内胆管结石相关肝内胆管癌中的表达及意义 [J]. 中国普通外科杂志, 2017, 26(2): 190-198.
Cite this article as: LIU Zhi, CHEN Yongzhi, SHU Jian, FU Yang, WANG Jun, LU Pin, ZHANG Honghui, ZHOU Zheng . Expressions of annexin A2, E-cadherin and vimentin in hepatolithiasis-associated intrahepatic cholangiocarcinoma and their significance [J]. Chin J Gen Surg, 2017, 26(2): 190-198.