基础研究(Basic Research)

长链非编码RNA在脂多糖刺激的大鼠肠巨噬细胞中的差异表达

Published at: 2017年第26卷第4期

刘路路 1 , 邹松 1 , 钱小宝 1 , 陈吉祥 1 , 瞿建国 1 , 崔磊 1 , 张建新 1 , 党胜春 1
1 江苏大学附属医院 普通外科,江苏 镇江 212001
通讯作者 胜春 党 Email: dscgu@163.com
DOI: 10.3978/j.issn.1005-6947.10.3978/j.issn.1005-6947.2017.04.014
基金:
国家自然科学基金资助项目 81070287

摘要

目的:探讨脂多糖(LPS)诱导的肠巨噬细胞炎症反应与长链非编码RNA(lncRNA)表达的关系。方法:分离培养大鼠肠巨噬细胞,用lncRNA表达谱芯片技术检测LPS处理的大鼠肠巨噬细胞(实验组)和无处理的大鼠肠巨噬细胞(对照组)之间差异表达的lncRNA,并用分层聚类的方法分析了基因芯片数据;构建差异表达的lncRNA-mRNA共表达网络,利用GO分析和通路分析预测其生物学功能;最后用RT-qPCR对部分差异表达的lncRNA进行验证。结果:与对照组比较,实验组共检测出357个差异表达的lncRNA(差异表达倍数>1.5),其中上调的有245个,下调的有112个。GO分析和通路分析显示差异表达的lncRNA参与多种生物过程,包括炎症反应,免疫应答和细胞凋亡,其中lncRNA NONMMUT024673、NONMMUT047081在网络中可能起重要作用。RT-qPCR验证NONMMUT024673、NONMMUT047081的表达,结果与基因芯片数据一致。结论:LPS可诱导大鼠肠巨噬细胞lncRNA表达改变,这些差异表达的lncRNA可能参与调控LPS诱导的肠巨噬细胞的炎症反应。


Differential expression of lncRNAs in rat intestinal macrophages with lipopolysaccharide stimulation

Abstract

Objective: To investigate the relationship between the expressions of long non-coding RNA (lncRNAs) and the inflammatory responses induced by lipopolysaccharide (LPS) in intestinal macrophages. Methods: Rat intestinal macrophages were isolated and cultured. The differentially expressed lncRNAs between LPS treated (experimental group) and untreated rat intestinal macrophages (control group) were determined by lncRNA expression microarray, and then the data from microarray were analyzed by hierarchical clustering method. The lncRNA-mRNA co-expression network was constructed, and then the biological function prediction was performed by GO analysis and pathway analysis. Finally, some differentially expressed lncRNAs detected by microarray were verified by RT-qPCR. Results: In experimental group compared with control group, a total of 357 differentially expressed lncRNAs (fold change >1.5) was identified, in which, 245 lncRNAs were up-regulated and 112 lncRNAs were down-regulated. GO analysis and pathway analysis indicated that these differentially expressed lncRNAs were involved in many biological processes that included in the inflammatory response, immune responses and apoptosis, and among them, the lncRNA NONMMUT024673 and NONMMUT047081 were probably most important in the network. The expressions of NONMMUT024673 and NONMMUT047081 examined by RT-qPCR were consistent with those obtained by microarray. Conclusion: LPS can elicit lncRNA differential expression in rat intestinal macrophages, and these differentially expressed lncRNAs may participate in the regulation of the inflammatory response intestinal macrophages induced by LPS.


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引用

引用本文: 路路 刘, 松 邹, 小宝 钱, 吉祥 陈, 建国 瞿, 磊 崔, 建新 张, 胜春 党. 长链非编码RNA在脂多糖刺激的大鼠肠巨噬细胞中的差异表达 [J]. 中国普通外科杂志, 2017, 26(4): 488-493.
Cite this article as: LIU Lulu, ZOU Song, QIAN Xiaobao, CHEN Jixiang, QU Jian'guo, CUI Lei, ZHANG Jianxin, DANG Shengchun . Differential expression of lncRNAs in rat intestinal macrophages with lipopolysaccharide stimulation [J]. Chin J Gen Surg, 2017, 26(4): 488-493.