基础研究(Basic Research)

miR-567在胰腺癌细胞中的表达及其作用机制

Published at: 2017年第26卷第9期

张婷 1 , 赵顺玉 1 , 孔双喜 1
1 华中科技大学同济医学院附属武汉中心医院 肿瘤科,湖北 武汉 430014
通讯作者 双喜 孔 Email: beidouxing0402@163.com
DOI: 10.3978/j.issn.1005-6947.10.3978/j.issn.1005-6947.2017.09.010
基金:

摘要

目的:探讨miR-567在胰腺癌细胞中的表达及其作用。方法:采用qRT-PCR检测正常胰腺导管上皮细胞系HPDE6-C7及胰腺癌细胞系Panc-1、AsPC-1、HPAC、BxPC-3中miR-567表达。Panc-1细胞转染miR-567过表达慢病毒载体后,分别用CCK-8法、流式细胞术、划痕愈合实验、qRT-PCR、Western blot法检测细胞增殖、凋亡及迁移能力,以及KPNA4 mRNA与蛋白的表达、凋亡相关蛋白表达的变化。结果:miR-567在胰腺癌细胞系Panc-1、AsPC-1、HPAC、BxPC-3中的表达水平均明显低于正常胰腺导管上皮细胞系HPDE6-C7(均P<0.05);miR-567慢病毒转染Panc-1细胞后,增殖能力明显减弱,凋亡率明显增加,划痕愈合率明显降低、KPNA4 mRNA与蛋白表达明显下调、而caspase-3及Bax蛋白表达明显上调(均P<0.05)。结论:miR-567在胰腺癌细胞中表达降低,升高其表达可抑制胰腺癌细胞的生长与迁移能力,其机制可能与下调KPNA4并上调凋亡相关蛋白表达有关。


MiR-567 expression in pancreatic carcinoma cells and its action mechanism

Abstract

Objective: To investigate the miR-567 expression in pancreatic cancer cells and its function. Methods: The miR-567 expressions in normal pancreatic duct epithelial cell line HPDE6-C7 and pancreatic carcinoma cell line Panc-1, AsPC-1, HPAC and BxPC-3 were determined by qRT-PCR. The miR-567 overexpression lentivirus vectors were transfected into the Panc-1 cells, and then, the changes in proliferation, apoptosis and migration ability as well as expressions of KPN4 mRNA and protein and apoptosis-associated proteins were analyzed by CCK-8 assay, flow cytometry, scratch wound healing assay, qRT-PCR and Western blot, respectively. Results: The miR-567 expressions in all the pancreatic cancer cell lines were significantly lower than that in normal pancreatic duct epithelial HPDE6-C7 cells (all P<0.05). In Panc-1 cells after transfection with miR-567 overexpression lentivirus vectors, the proliferation was decreased, apoptosis rate was increased, scratch healing rate was reduced, and both KPNA4 mRNA and protein expressions were down-regulated, while the caspase-3 and Bax protein expressions were up-regulated significantly (all P<0.05). Conclusion: MiR-567 expression is decreased in pancreatic cancer cells, and up-regulating its expression can inhibit the growth and migration ability of pancreatic cancer cells, and the mechanism may possibly be associated with its decreasing KPNA4 expression and increasing the expressions of apoptosis-associated proteins.


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引用

引用本文: 婷 张, 顺玉 赵, 双喜 孔. miR-567在胰腺癌细胞中的表达及其作用机制[J]. 中国普通外科杂志, 2017, 26(9): 1141-1147.
Cite this article as: ZHANG Ting, ZHAO Shunyu, KONG Shuangxi . MiR-567 expression in pancreatic carcinoma cells and its action mechanism [J]. Chin J Gen Surg, 2017, 26(9): 1141-1147.