基础研究(Basic Research)

miR-26b的表达及其与Foxf2相互作用在乳腺癌细胞生物学行为中的作用

Published at: 2017年第26卷第11期

王雷 1 , 陈传波 1 , 马明德 1
1 河南大学附属淮河医院 乳腺外科,河南 开封 475001
通讯作者 明德 马 Email: wanglei198101@sina.com
DOI: 10.3978/j.issn.1005-6947.2017.11.011
基金:

摘要

目的:探讨miR-26b在乳腺癌细胞中的表达及其对乳腺癌细胞生物学行为的的影响。方法:比较正常乳腺细胞系MCF-10A及乳腺癌细胞系MCF-7中miR-26b的表达差异。以无处理的MCF-7细胞为空白对照,分别检测MCF-7细胞转染miR-26b 模拟物(miR-26b 组)、空质粒(阴性对照组)后的miR-26b表达与增殖、迁移、侵袭能力,以及Foxf2的mRNA与蛋白表达的变化。用双荧光素酶报告系统检测miR-26b对MCF-7细胞中Foxf2转录活性的影响。结果:miR-26b在MCF-7细胞中的表达水平明显低于MCF-10A细胞(P<0.05)。与空白对照组和阴性对照组比较,miR-26b组miR-26b mRNA表达水平明显升高、细胞增殖迁移、侵袭能力明显降低,Foxf2的mRNA和蛋白表达量均明显下调(P<0.05)。转染miR-26b模拟物后,MCF-7细胞中Foxf2-3'UTR的转录活性明显抑制(P<0.05)。结论:miR-26b在乳腺癌细胞中表达降低、增加其表达能抑制乳腺癌细胞的恶性生物学行为,机制可能与其下调Foxf2的表达有关。


Effects of miR-26b expression and its interaction with Foxf2 on the biological behaviors in breast cancer cells

Abstract

Objective: To investigate the miR-26b expression and its influence on the biological behaviors in breast cancer cells. Methods: The differences of miR-26b expressions in normal breast cell line MCF-10A and breast cancer cell line MCF-7 were compared. Using untreated MCF-7 cells as blank control, the changes in miR-26b expression, abilities of proliferation, migration and invasion, and Foxf2 mRNA and protein expressions in MCF-7 cells after transfection with miR-26b mimics (miR-26b) group or empty plasmid (negative control group) were determined. The influence of miR-26b on transcriptional activity of Foxf2 in MCF-7 cells was determined by dual-luciferase reporter assay system. Results: The miR-26b expression level in MCF-7 cells was significantly lower than that in MCF-10A cells (P<0.05). In miR-26b group compared with the blank control group or negative control group, the miR-26b expression level was significantly increased, the abilities of proliferation, migration and invasion were significantly decreased, and the mRNA and protein expression levels of Foxf2 were significantly down-regulated (all P<0.05). The transcriptional activity of Foxf2-3'UTR was significantly inhibited in MCF-7 cells after miR-26b mimics transfection (P<0.05). Conclusion: MiR-26b expression is decreased in breast cancer cells, increasing its expression can suppress the malignant biological behaviors of breast cancer cells, and the mechanism may be related to its down-regulating Foxf2 expression.


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引用

引用本文: 雷 王, 传波 陈, 明德 马. miR-26b的表达及其与Foxf2相互作用在乳腺癌细胞生物学行为中的作用[J]. 中国普通外科杂志, 2017, 26(11): 1439-1446.
Cite this article as: WANG Lei, CHEN Chuanbo, MA Mingde . Effects of miR-26b expression and its interaction with Foxf2 on the biological behaviors in breast cancer cells[J]. Chin J Gen Surg, 2017, 26(11): 1439-1446.