基础研究(Basic Research)

索拉非尼对人肝癌细胞生长抑制作用的体外研究

Published at: 2015年第24卷第7期

李克跃 1 , 石承先 1 , 汤可立 1
1 贵州省人民医院 肝胆外科,贵州 贵阳 550002
通讯作者 承先 石 Email: ChengxianL@aliyun.com
DOI: 10.3978/j.issn.1005-6947.10.3978/j.issn.1005-6947.2015.07.008
基金:

摘要

目的:探讨索拉非尼对人肝癌(HCC)细胞体外生长及肿瘤相关基因表达的影响。方法: 用不同浓度(0、5、10、20 μmol/L) 索拉非尼作用人HCC 细胞(Hep3B2.1-7)24 h 后, 用CCK-8 法观察细胞的增殖情况, 并分别用real-time PCR 与Western blot 检测各组HCC 细胞p53、PTEN、TGF-β1 的mRNA 与蛋白表达。结果:与0 μmol/L 索拉非尼处理组(对照组)比较,其余各浓度索拉非尼处理组HCC 细胞增殖均明显降低(均P<0.05);p53、PTEN 的mRNA 与蛋白表达明显升高,而TGF-β1 的mRNA 与蛋白表达明显降低(均P<0.05),以上作用均呈一定的浓度依赖性。结论:索拉非尼对HCC 细胞体外生长有抑制作用,其作用机制与调控肿瘤相关基因的表达有关。


Role of sorafenib on inhibition of growth of human hepatocellular carcinoma cells in vitro

Abstract

Objective: To investigate the influence of sorafenib on growth and expressions of tumor-related genes in human hepatocellular carcinoma (HCC) cells in vitro. Methods: Human HCC cells (Hep3B2.1-7) were exposed to different concentrations (0, 5, 10, and 20 μmol/L) of sorafenib for 24 h, and then, the cell proliferation was analyzed by CCK-8 assay, and the mRNA and protein expressions of p53, PTEN and TGF-β1 in the HCC cells were determined by real-time PCR and Western blot, respectively. Results: Compared with the group of cells with 0 μmol/L sorafenib treatment (control group), in the other groups of cells with sorafenib treatment, the cell proliferation rates were all significantly decreased (all P<0.05); the mRNA and protein expressions of p53 and PTEN were significantly increased, while the mRNA and protein expressions of TGF-β1 were significantly decreased (all P<0.05). All these effects of sorafenib showed a certain concentration-dependent manner. Conclusion: Sorafenib can inhibit the growth of human HCC cells in vitro, and the mechanism may be attributed to its regulating the expressions of tumor-related genes.


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引用

引用本文: 克跃 李, 承先 石, 可立 汤. 索拉非尼对人肝癌细胞生长抑制作用的体外研究[J]. 中国普通外科杂志, 2015, 24(7): 958-962.
Cite this article as: LI Keyue, SHI Chengxian, TANG Keli . Role of sorafenib on inhibition of growth of human hepatocellular carcinoma cells in vitro[J]. Chin J Gen Surg, 2015, 24(7): 958-962.