基础研究(Basic Research)

腹腔镜犬胆道梗阻模型研究

Published at: 2015年第24卷第2期

薛飞 1,2,3 , 吕毅 1,2,3 , 虢宏昌 4,5 , 马锋 2,3 , 陆建文 1,2,3 , 李建鹏 1,2,3 , 白纪刚 1,2,3 , 刘亚雄 4,5
1 西安交通大学第一附属医院 肝胆外科,陕西 西安710061
2 陕西省再生医学与外科工程技术研究中心,陕西 西安 710061
3 西安交通大学先进外科技术与工程研究所,陕西 西安 710061
4 西安工程大学机电工程学院,陕西 西安 710048
5 西安交通大学机械制造系统工程国家重点实验室,陕西 西安 710049
通讯作者 毅 吕 Email: luyi169@126.com
DOI: 10.3978/j.issn.1005-6947.10.3978/j.issn.1005-6947.2015.02.011
基金:
国家自然科学基金资助项目 51275387
国家自然科学基金科学仪器专项基金资助项目 81127005

摘要

目的:探讨应用腹腔镜微创技术制作胆道梗阻模型的动物模型的可行性。方法:用6 只杂种犬进行腹腔镜下胆总管结扎手术,记录手术及术后情况,检测术前与术后(3、7、10 d)白细胞(WBC)、肝功能指标、C 反应蛋白(C-RP)、血清降钙素原(PCT)水平,术后10 d 观测胆道扩张情况及肝脏病理学改变。结果:总手术时间、麻醉苏醒时间、首次进食与排便时间分别为(39.17±3.4)min、(59.17±12.8)min、(3.5±0.63)h、1 d。WBC 水平在术后3 d 明显升高(P<0.05),但随后快速恢复正常(P>0.05);术后C-RP 与PCT 保持正常水平(均P>0.05);术后转氨酶水平先升后降,但均明显高于术前(均P<0.05);胆红素水平呈持续升高(均P<0.05)。术后10 d,肉眼可见胆总管扩张明显,病理学显示微胆管扩张。结论:利用腹腔镜技术建立犬胆道梗阻模型简便、微创、可行,该造模方法为今后的实验研究提供了便利。


Experimental study on canine biliary obstruction using laparoscopic technique

Abstract

Objective: To investigate the feasibility of using laparoscopic minimally invasive technique to create animal model of biliary occlusion. Methods: Six mongrel dogs underwent laparoscopic common bile duct (CBD) ligation. The operative and postoperative variables were recorded, the preoperative and postoperative (day 3, 7 and 10) levels of white blood cells, liver function indexes, C-reactive protein (C-RP) and procalcitonin (PCT) were determined, and the CBD dilation and liver pathological changes were observed on postoperative day 10. Results: The overall operative time, time to recovery from anesthesia, time to first food intake and defecation was (39.17±3.4) min, (59.17±12.8) min, (3.5±0.63) h and 1 d, respectively. The WBC level was significantly elevated at postoperative day 3 (P<0.05), but soon recovered (P>0.05); the postoperative C-RP and PCT levels remained normal (all P>0.05); the postoperative transaminase levels were initially increased and then decreased slowly, but all were significantly higher than those of preoperative levels (all P<0.05); the postoperative bilirubin levels showed continuous increase (all P<0.05). Obvious CBD dilation was seen macroscopically and pathological examination showed marked dilation of the bile capillaries. Conclusion: Establishment of canine model of biliary occlusion using laparoscopic technique is simple, minimally invasive and feasible, and this method of model creation may facilitate future experimental studies.


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引用

引用本文: 飞 薛, 毅 吕, 宏昌 虢, 锋 马, 建文 陆, 建鹏 李, 纪刚 白, 亚雄 刘. 腹腔镜犬胆道梗阻模型研究[J]. 中国普通外科杂志, 2015, 24(2): 211-215.
Cite this article as: XUE Fei, LU Yi, GUO Hongchang, MA Feng, LU Jianwen, LI Jianpeng, BAI Jigang, LIU Yaxiong . Experimental study on canine biliary obstruction using laparoscopic technique[J]. Chin J Gen Surg, 2015, 24(2): 211-215.