目的：探讨应用腹腔镜微创技术制作胆道梗阻模型的动物模型的可行性。方法：用6 只杂种犬进行腹腔镜下胆总管结扎手术，记录手术及术后情况，检测术前与术后（3、7、10 d）白细胞（WBC）、肝功能指标、C 反应蛋白（C-RP）、血清降钙素原（PCT）水平，术后10 d 观测胆道扩张情况及肝脏病理学改变。结果：总手术时间、麻醉苏醒时间、首次进食与排便时间分别为（39.17±3.4）min、（59.17±12.8）min、（3.5±0.63）h、1 d。WBC 水平在术后3 d 明显升高（P<0.05），但随后快速恢复正常（P>0.05）；术后C-RP 与PCT 保持正常水平（均P>0.05）；术后转氨酶水平先升后降，但均明显高于术前（均P<0.05）；胆红素水平呈持续升高（均P<0.05）。术后10 d，肉眼可见胆总管扩张明显，病理学显示微胆管扩张。结论：利用腹腔镜技术建立犬胆道梗阻模型简便、微创、可行，该造模方法为今后的实验研究提供了便利。
Experimental study on canine biliary obstruction using laparoscopic technique
Objective: To investigate the feasibility of using laparoscopic minimally invasive technique to create animal model of biliary occlusion. Methods: Six mongrel dogs underwent laparoscopic common bile duct (CBD) ligation. The operative and postoperative variables were recorded, the preoperative and postoperative (day 3, 7 and 10) levels of white blood cells, liver function indexes, C-reactive protein (C-RP) and procalcitonin (PCT) were determined, and the CBD dilation and liver pathological changes were observed on postoperative day 10. Results: The overall operative time, time to recovery from anesthesia, time to first food intake and defecation was (39.17±3.4) min, (59.17±12.8) min, (3.5±0.63) h and 1 d, respectively. The WBC level was significantly elevated at postoperative day 3 (P<0.05), but soon recovered (P>0.05); the postoperative C-RP and PCT levels remained normal (all P>0.05); the postoperative transaminase levels were initially increased and then decreased slowly, but all were significantly higher than those of preoperative levels (all P<0.05); the postoperative bilirubin levels showed continuous increase (all P<0.05). Obvious CBD dilation was seen macroscopically and pathological examination showed marked dilation of the bile capillaries. Conclusion: Establishment of canine model of biliary occlusion using laparoscopic technique is simple, minimally invasive and feasible, and this method of model creation may facilitate future experimental studies.