基础研究(Basic Research)

表皮生长因子受体抑制剂联合5- 氟尿嘧啶对胆管癌细胞增殖与迁移、侵袭能力的影响

Published at: 2015年第24卷第8期

曾杰宏 1 , 彭思远 1 , 谢博文 1 , 刘铮凯 1 , 陈晨 1 , 李浩 1 , 蒋波 1
1 湖南师范大学第一附属医院/ 湖南省人民医院 肝胆外科,湖南 长沙 410006
通讯作者 波 蒋 Email: jiangbo@medmail.com.cn
DOI: 10.3978/j.issn.1005-6947.10.3978/j.issn.1005-6947.2015.08.009
基金:
湖南省卫生厅医药卫生科研计划资助项目 B2012-088
湖南省科学技术厅科技计划资助项目 2014FJ3033

摘要

目的:探讨表皮生长因子受体(EGFR)抑制剂AG1478 联合5- 氟尿嘧啶(5-FU)对胆管癌细胞体外增殖、迁移和侵袭的影响。方法:体外培养人胆管癌QBC939 细胞,分别用AG1478(10 μmol/L)、5-FU(100 mg/L)以及联合两药处理24 h 后,分别采用CCK-8 法、划痕试验、Matrigel 侵袭小室法检测细胞增殖、迁移及侵袭情况。结果:AG1478 与5-FU 单独作用后,QBC939 细胞增殖、迁移和侵袭能力均明显降低(均P<0.05),而与任一单独用药比较,联合用药对细胞增殖、迁移和侵袭能力的抑制作用均明显增强(均P<0.05)。结论:EGFR 抑制剂与5-FU 联合应用可能是抑制胆管癌细胞生长、迁移和侵袭有效方法。


Effects of epidermal growth factor receptor inhibitor plus 5-fluorouracil on proliferation, migration and invasiveness of cholangiocarcinoma cells

Abstract

Objective: To investigate the effects of epidermal growth factor receptor (EGFR) inhibitor AG1478 in combination with 5-fluorouracil (5-FU) on proliferation, migration and invasiveness of cholangiocarcinoma cells in vitro. Methods: Human cholangiocarcinoma QBC939 cells were cultured in vitro and exposed to AG1478 (10 μmol/L) and 5-FU (100 mg/L) alone or in combination for 24 h. The cell proliferation, migration and invasiveness were measured by CCK-8 assay, wound-scratch assay and Matrigel invasion chamber assay, respectively. Results: The abilities of proliferation, migration and invasion of QBC939 cells were significantly decreased after either lone AG1478 or 5-FU treatment (all P<0.05), and above effects were further enhanced by the combined treatment compared with either lone drug treatment (all P<0.05). Conclusion: Combination use of EGFR inhibitor and 5-FU may be an effective method for inhibiting the growth, migration and invasiveness of cholangiocarcinoma cells.


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引用

引用本文: 杰宏 曾, 思远 彭, 博文 谢, 铮凯 刘, 晨 陈, 浩 李, 波 蒋. 表皮生长因子受体抑制剂联合5- 氟尿嘧啶对胆管癌细胞增殖与迁移、侵袭能力的影响[J]. 中国普通外科杂志, 2015, 24(8): 1102-1106.
Cite this article as: ZENG Jiehong, PENG Siyuan, XIE Bowen, LIU Zhengkai, CHEN Chen, LI Hao, JIANG Bo . Effects of epidermal growth factor receptor inhibitor plus 5-fluorouracil on proliferation, migration and invasiveness of cholangiocarcinoma cells[J]. Chin J Gen Surg, 2015, 24(8): 1102-1106.