目的：探讨表皮生长因子受体（EGFR）抑制剂AG1478 联合5- 氟尿嘧啶（5-FU）对胆管癌细胞体外增殖、迁移和侵袭的影响。方法：体外培养人胆管癌QBC939 细胞，分别用AG1478（10 μmol/L）、5-FU（100 mg/L）以及联合两药处理24 h 后，分别采用CCK-8 法、划痕试验、Matrigel 侵袭小室法检测细胞增殖、迁移及侵袭情况。结果：AG1478 与5-FU 单独作用后，QBC939 细胞增殖、迁移和侵袭能力均明显降低（均P<0.05），而与任一单独用药比较，联合用药对细胞增殖、迁移和侵袭能力的抑制作用均明显增强（均P<0.05）。结论：EGFR 抑制剂与5-FU 联合应用可能是抑制胆管癌细胞生长、迁移和侵袭有效方法。
Effects of epidermal growth factor receptor inhibitor plus 5-fluorouracil on proliferation, migration and invasiveness of cholangiocarcinoma cells
Objective: To investigate the effects of epidermal growth factor receptor (EGFR) inhibitor AG1478 in combination with 5-fluorouracil (5-FU) on proliferation, migration and invasiveness of cholangiocarcinoma cells in vitro. Methods: Human cholangiocarcinoma QBC939 cells were cultured in vitro and exposed to AG1478 (10 μmol/L) and 5-FU (100 mg/L) alone or in combination for 24 h. The cell proliferation, migration and invasiveness were measured by CCK-8 assay, wound-scratch assay and Matrigel invasion chamber assay, respectively. Results: The abilities of proliferation, migration and invasion of QBC939 cells were significantly decreased after either lone AG1478 or 5-FU treatment (all P<0.05), and above effects were further enhanced by the combined treatment compared with either lone drug treatment (all P<0.05). Conclusion: Combination use of EGFR inhibitor and 5-FU may be an effective method for inhibiting the growth, migration and invasiveness of cholangiocarcinoma cells.