目的：研究二氢杨梅素（DHM）对肝癌细胞黏附、侵袭和迁移能力的影响及其可能机制。 方法：用不同浓度DHM处理肝癌MHCC97L细胞后，分别检测细胞的黏附能力、迁移与侵袭能力，以及E-cadherin、MMP-2、MMP-9和VEGF蛋白表达。 结果：与空白对照细胞比较，DHM处理后的MHCC97L细胞黏附力明显降低、侵袭与迁移力明显减弱（均P<0.05）；E-cadherin表达明显上调，而MMP-9、VEGF蛋白表达明显下调的水平（均P<0.05），但MMP-2蛋白的表达无明显改变（均P>0.05）。 结论：DHM可能通过调控E-cadherin、MMP-9和VEGF蛋白的表达抑制肝癌细胞的黏附、迁移和侵袭。
Inhibitory effect of dihydromyricetin on adhesion, invasion and migration in hepatocellular carcinoma cells and the mechanism
Objective: To investigate the effect of dihydromyricetin (DHM) on adhesion, invasion and migration abilities of hepatocellular carcinoma (HCC) cells and the possible mechanisms. Methods: HCC MHCC97L cells were exposed to different concentrations of DHM, and then, the adhesion, invasion and migration abilities of the cells were examined, and the protein expressions of E-cadherin, MMP-2, MMP-9 and VEGF were also determined. Results: In MHCC97L cells treated with DHM compared with blank control cells, the abilities of adhesion, invasion and migration were all significantly deceased (all P<0.05), the E-cadherin expression was upregulated, and the MMP-9 and VEGF expressions were downregulated significantly, but the MMP-2 expression showed no significant change (P>0.05). Conclusion: DHM can inhibit the adhesion, invasion and migration of HCC cells by regulation of E-cadherin, MMP-9 and VEGF expressions.