基础研究(Basic Research)

二氢杨梅素对肝癌细胞黏附、侵袭及迁移的抑制作用及机制

Published at: 2015年第24卷第9期

邱志东 1 , 罗芳兰 2 , 舒洋 2 , 温小军 2 , 张庆余 3 , 廖雅琳 4 , 黎然 3 , 缪辉来 3
1 广东省深圳市第七人民医院 普通外科,广东 深圳 518000
2 广东医学院研究生学院,广东 湛江 524000
3 广东医学院附属医院 肝胆外科,广东 湛江524000
4 广东医学院第一临床学院,广东 湛江 524000
通讯作者 辉来 缪 Email: gandanwaike2007@126.com
DOI: 10.3978/j.issn.1005-6947.10.3978/j.issn.1005-6947.2015.09.011
基金:

摘要

目的:研究二氢杨梅素(DHM)对肝癌细胞黏附、侵袭和迁移能力的影响及其可能机制。 方法:用不同浓度DHM处理肝癌MHCC97L细胞后,分别检测细胞的黏附能力、迁移与侵袭能力,以及E-cadherin、MMP-2、MMP-9和VEGF蛋白表达。 结果:与空白对照细胞比较,DHM处理后的MHCC97L细胞黏附力明显降低、侵袭与迁移力明显减弱(均P<0.05);E-cadherin表达明显上调,而MMP-9、VEGF蛋白表达明显下调的水平(均P<0.05),但MMP-2蛋白的表达无明显改变(均P>0.05)。 结论:DHM可能通过调控E-cadherin、MMP-9和VEGF蛋白的表达抑制肝癌细胞的黏附、迁移和侵袭。


Inhibitory effect of dihydromyricetin on adhesion, invasion and migration in hepatocellular carcinoma cells and the mechanism

Abstract

Objective: To investigate the effect of dihydromyricetin (DHM) on adhesion, invasion and migration abilities of hepatocellular carcinoma (HCC) cells and the possible mechanisms. Methods: HCC MHCC97L cells were exposed to different concentrations of DHM, and then, the adhesion, invasion and migration abilities of the cells were examined, and the protein expressions of E-cadherin, MMP-2, MMP-9 and VEGF were also determined. Results: In MHCC97L cells treated with DHM compared with blank control cells, the abilities of adhesion, invasion and migration were all significantly deceased (all P<0.05), the E-cadherin expression was upregulated, and the MMP-9 and VEGF expressions were downregulated significantly, but the MMP-2 expression showed no significant change (P>0.05). Conclusion: DHM can inhibit the adhesion, invasion and migration of HCC cells by regulation of E-cadherin, MMP-9 and VEGF expressions.


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引用

引用本文: 志东 邱, 芳兰 罗, 洋 舒, 小军 温, 庆余 张, 雅琳 廖, 然 黎, 辉来 缪. 二氢杨梅素对肝癌细胞黏附、侵袭及迁移的抑制作用及机制[J]. 中国普通外科杂志, 2015, 24(9): 1263-1268.
Cite this article as: QIU Zhidong, LUO Fanglan, SHU Yang, WEN Xiaojun, ZHANG Qingyu, LIAO Yalin, LI Ran, MIAO Huilai . Inhibitory effect of dihydromyricetin on adhesion, invasion and migration in hepatocellular carcinoma cells and the mechanism[J]. Chin J Gen Surg, 2015, 24(9): 1263-1268.