@article{PW4521,
author = {顺崇 肖 和 汉传 罗 和 俊仕 覃},
title = {miR-204对TFAM的靶向调控作用及其对乳腺癌细胞生长与增殖的影响
},
journal = {中国普通外科杂志},
volume = {25},
number = {11},
year = {2016},
keywords = {},
abstract = {目的:探讨乳腺癌细胞中miR-204对线粒体转录因子A(TFAM)的靶向调控作用及其与细胞生长、增殖的关系。方法:将人乳腺癌MDA-MB-231细胞分别转染miR-204模拟物或miR-204抑制物,用real-time PCR和Western blot分别检测miR-204与TFAM蛋白的表达;构建荧光酶报告基因质粒(mut-TFAM/wt-TFAM),将其与miR-204模拟物或miR-204抑制物共转染MDA-MB-231细胞后检测荧光酶活性变化;构建pcDNA3.1/TFAM质粒,将其单独或与miR-204模拟物共转染MDA-MB-231细胞后检测TFAM蛋白表达,并用MTT法和BrdU法检测细胞生长与增殖情况。结果:MDA-MB-231细胞转染miR-204模拟物后miR-204的表达明显升高,而TFAM蛋白表达明显降低,转染miR-204抑制物后则呈反向变化(均P},
url = {https://pw.amegroups.com/article/view/4521}
}