CARMA3 在胆管癌细胞中的表达及功能
| 作者: |
1贺林华,
2华颂文,
3李劲东
1 湖南司法警官职业学院，湖南 长沙 410131 2 中南大学湘雅二医院 普通外科，湖南 长沙 410011 3 中南大学湘雅医院 普通外科，湖南 长沙 410008 |
| 通讯: |
华颂文
Email: 58398285@qq.com |
| DOI: | 10.3978/.2018.08.009 |
摘要
目的：探讨接头蛋白 CARMA3 在胆管癌细胞中的表达及功能。
方法：用 qRT-PCR 技术检测胆管癌 HUCCT1 细胞与 RBE 细胞以及正常胆管细胞 HIBEC 中 CARMA3的 mRNA 表达。用 siRNA 技术沉默 HUCCT1 细胞与 RBE 细胞中 CARMA3 的表达后，分别通过 CCK-8法、流式细胞术、Transwell 实验检测细胞增殖、凋亡、细胞周期以及转移和侵袭能力的变化。
结果：两种胆管癌细胞中 CARMA3 的 mRNA 表达水平均明显高于正常胆管细胞 HIBEC（HUCCT1 vs. HIBEC：t=5.321，P=0.011；RBE vs. HIBEC：t=5.932，P=0.008）。 沉默 CARMA3 的表达后，两种胆管癌细胞的增殖、转移和细胞侵袭能力被明显抑制，G1 期阻滞明显增加，细胞凋亡率明显升高（均P<0.05）。
结论：CARMA3 在胆管癌细胞中表达上调，其作用可能与促进细胞增殖、侵袭、转移并抑制细胞凋亡。
关键词：
胆管肿瘤；CARD 信号接头蛋白质类；细胞增殖；细胞凋亡；肿瘤侵润
方法：用 qRT-PCR 技术检测胆管癌 HUCCT1 细胞与 RBE 细胞以及正常胆管细胞 HIBEC 中 CARMA3的 mRNA 表达。用 siRNA 技术沉默 HUCCT1 细胞与 RBE 细胞中 CARMA3 的表达后，分别通过 CCK-8法、流式细胞术、Transwell 实验检测细胞增殖、凋亡、细胞周期以及转移和侵袭能力的变化。
结果：两种胆管癌细胞中 CARMA3 的 mRNA 表达水平均明显高于正常胆管细胞 HIBEC（HUCCT1 vs. HIBEC：t=5.321，P=0.011；RBE vs. HIBEC：t=5.932，P=0.008）。 沉默 CARMA3 的表达后，两种胆管癌细胞的增殖、转移和细胞侵袭能力被明显抑制，G1 期阻滞明显增加，细胞凋亡率明显升高（均P<0.05）。
结论：CARMA3 在胆管癌细胞中表达上调，其作用可能与促进细胞增殖、侵袭、转移并抑制细胞凋亡。
CARMA3 expression in cholangiocarcinoma cells and its function
CorrespondingAuthor:HUA Songwen Email: 58398285@qq.com
Abstract
Objective: To investigate the expression of the adaptor protein CARMA3 in cholangiocarcinoma cells and its function.
Methods: The expressions of CARMA3 mRNA in cholangiocarcinoma HUCCT1 and RBE cells as well as in normal biliary epithelia cell line HIBEC were determined by qRT-PCR. After silencing the expression of CARMA3 with siRNA technique, the changes in proliferation, apoptosis, cell cycle and abilities of migration and invasion in HUCCT1 and RBE cells were examined by CCK-8 assay, fl ow cytometry and Transwell assay, respectively.
Results: Th e expression levels of CARMA3 mRNA were signifi cantly increased in both cholangiocarcinoma cell lines compared with normal biliary epithelia cell line HIBEC (HUCCT1 vs. HIBEC: t=5.321, P=0.011; RBE vs. HIBEC: t=5.932, P=0.008). In both cholangiocarcinoma cell lines after silencing the expression of CARMA3, the abilities of proliferation, metastasis and invasion were significantly inhibited, and the G1-phase arrest and apoptosis rates were significantly increased (all P<0.05).
Conclusion: CARMA3 is up-regulated in cholangiocarcinoma cells, and its actions may be associated with promoting cell proliferation, migration and invasion, and inhibiting cell apoptosis.
Keywords：
Bile Duct Neoplasms; CARD Signaling Adaptor Proteins; Cell Proliferation; Apoptosis; Neoplasm Invasiveness
Methods: The expressions of CARMA3 mRNA in cholangiocarcinoma HUCCT1 and RBE cells as well as in normal biliary epithelia cell line HIBEC were determined by qRT-PCR. After silencing the expression of CARMA3 with siRNA technique, the changes in proliferation, apoptosis, cell cycle and abilities of migration and invasion in HUCCT1 and RBE cells were examined by CCK-8 assay, fl ow cytometry and Transwell assay, respectively.
Results: Th e expression levels of CARMA3 mRNA were signifi cantly increased in both cholangiocarcinoma cell lines compared with normal biliary epithelia cell line HIBEC (HUCCT1 vs. HIBEC: t=5.321, P=0.011; RBE vs. HIBEC: t=5.932, P=0.008). In both cholangiocarcinoma cell lines after silencing the expression of CARMA3, the abilities of proliferation, metastasis and invasion were significantly inhibited, and the G1-phase arrest and apoptosis rates were significantly increased (all P<0.05).
Conclusion: CARMA3 is up-regulated in cholangiocarcinoma cells, and its actions may be associated with promoting cell proliferation, migration and invasion, and inhibiting cell apoptosis.