二氢杨梅素对肝癌细胞黏附、侵袭及迁移的抑制作用及机制
| 作者: |
1邱志东,
2罗芳兰,
2舒洋,
2温小军,
3张庆余,
4廖雅琳,
3黎然,
3缪辉来
1 广东省深圳市第七人民医院 普通外科，广东 深圳 518000 2 广东医学院研究生学院，广东 湛江 524000 3 广东医学院附属医院 肝胆外科，广东 湛江524000 4 广东医学院第一临床学院，广东 湛江 524000 |
| 通讯: |
缪辉来
Email: gandanwaike2007@126.com |
| DOI: | 10.3978/.10.3978/j.issn.1005-6947.2015.09.011 |
摘要
目的：研究二氢杨梅素（DHM）对肝癌细胞黏附、侵袭和迁移能力的影响及其可能机制。
方法：用不同浓度DHM处理肝癌MHCC97L细胞后，分别检测细胞的黏附能力、迁移与侵袭能力，以及E-cadherin、MMP-2、MMP-9和VEGF蛋白表达。
结果：与空白对照细胞比较，DHM处理后的MHCC97L细胞黏附力明显降低、侵袭与迁移力明显减弱（均P<0.05）；E-cadherin表达明显上调，而MMP-9、VEGF蛋白表达明显下调的水平（均P<0.05），但MMP-2蛋白的表达无明显改变（均P>0.05）。
结论：DHM可能通过调控E-cadherin、MMP-9和VEGF蛋白的表达抑制肝癌细胞的黏附、迁移和侵袭。
关键词：
癌，肝细胞；二氢杨梅素；肿瘤侵润
方法：用不同浓度DHM处理肝癌MHCC97L细胞后，分别检测细胞的黏附能力、迁移与侵袭能力，以及E-cadherin、MMP-2、MMP-9和VEGF蛋白表达。
结果：与空白对照细胞比较，DHM处理后的MHCC97L细胞黏附力明显降低、侵袭与迁移力明显减弱（均P<0.05）；E-cadherin表达明显上调，而MMP-9、VEGF蛋白表达明显下调的水平（均P<0.05），但MMP-2蛋白的表达无明显改变（均P>0.05）。
结论：DHM可能通过调控E-cadherin、MMP-9和VEGF蛋白的表达抑制肝癌细胞的黏附、迁移和侵袭。
Inhibitory effect of dihydromyricetin on adhesion, invasion and migration in hepatocellular carcinoma cells and the mechanism
CorrespondingAuthor:MIAO Huilai Email: gandanwaike2007@126.com
Abstract
Objective: To investigate the effect of dihydromyricetin (DHM) on adhesion, invasion and migration abilities of hepatocellular carcinoma (HCC) cells and the possible mechanisms.
Methods: HCC MHCC97L cells were exposed to different concentrations of DHM, and then, the adhesion, invasion and migration abilities of the cells were examined, and the protein expressions of E-cadherin, MMP-2, MMP-9 and VEGF were also determined.
Results: In MHCC97L cells treated with DHM compared with blank control cells, the abilities of adhesion, invasion and migration were all significantly deceased (all P<0.05), the E-cadherin expression was upregulated, and the MMP-9 and VEGF expressions were downregulated significantly, but the MMP-2 expression showed no significant change (P>0.05).
Conclusion: DHM can inhibit the adhesion, invasion and migration of HCC cells by regulation of E-cadherin, MMP-9 and VEGF expressions.
Keywords：
Methods: HCC MHCC97L cells were exposed to different concentrations of DHM, and then, the adhesion, invasion and migration abilities of the cells were examined, and the protein expressions of E-cadherin, MMP-2, MMP-9 and VEGF were also determined.
Results: In MHCC97L cells treated with DHM compared with blank control cells, the abilities of adhesion, invasion and migration were all significantly deceased (all P<0.05), the E-cadherin expression was upregulated, and the MMP-9 and VEGF expressions were downregulated significantly, but the MMP-2 expression showed no significant change (P>0.05).
Conclusion: DHM can inhibit the adhesion, invasion and migration of HCC cells by regulation of E-cadherin, MMP-9 and VEGF expressions.