内质网应激相关蛋白CHOP/GADD153 在结肠癌组织中的表达及意义
| 作者: |
1朱兴国,
2张俊强,
1王成宏,
1何慧菊,
2赵欢,
2陈晓宇
1 安徽医科大学第三附属医院/ 合肥市第一人民医院 普通外科，安徽 合肥230088 2 安徽医科大学 组织学与胚胎学教研室，安徽 合肥230032 |
| 通讯: |
陈晓宇
Email: chenxy@163.com |
| DOI: | 10.3978/.10.3978/j.issn.1005-6947.2015.04.011 |
| 基金: | 国家自然科学资金资助项目, 81373421 |
摘要
目的：探讨结肠癌组织内质网应激相关蛋白CHOP/GADD153 的表达，并分析其与临床病理特征的关系。 方法： 选择82 例结肠癌患者的结肠癌组织与相应的癌旁正常结肠组织（距癌组织>5 cm）制作蜡块 后构建组织芯片，分别用免疫组化法及Western blot 法检测CHOP/GADD153 蛋白的表达，并分析其 表达与患者临床病理特征分关系。 结果：免疫组化与Western blot 结果均显示，结肠癌组织CHOP/GADD153 蛋白的表达水平明显癌旁 正常结肠组织（P<0.05）；与临床病理特征的关系分析显示，结肠癌组织中CHOP/GADD153 蛋白的 表达水平随结肠癌组织分化程度的降低而增强（P<0.05），而与患者的年龄、性别、肿瘤的大小、 肿瘤的浸润深度和淋巴结转移等因素无关（均P>0.05）。 结论：结肠癌组织中CHOP/GADD153 蛋白的表达增高，并与结肠癌织的分化程度密切相关，提示内 质网应激可能参与了肿瘤分化的调控。
关键词：
结肠肿瘤
CHOP/GADD153
应激，内质网
组织阵列分析
Expression of endoplasmic reticulum stress-related protein CHOP/GADD153 in colon cancer tissue and its significance
CorrespondingAuthor:CHEN Xiaoyu Email: chenxy@163.com
Abstract
Objective: To investigate the expression of the endoplasmic reticulum stress (ERS)-related protein CHOP/ GADD153 in colon cancer and its relations with clinicopathologic characteristics. Methods: The specimens of colon cancer tissue and normal adjacent colonic tissue (>5 cm distance from cancer tissue) were taken from 82 colon cancer patients and casted into paraffin blocks for tissue microarray construction. The CHOP/GADD153 protein expression was detected by immunohistochemical staining and Western blot analysis respectively, and the relations of its expression with clinicopathologic characteristics of the patients were analyzed. Results: Both results of immunohistochemical and Western blot analysis showed that the expression level of CHOP/GADD153 protein in the colon cancer tissue was significantly higher than that in normal adjacent colonic tissue (P<0.05). The analysis of relationship between CHOP/GADD153 protein expression and clinicopathologic profiles indicated that the expression level of CHOP/GADD153 protein in colon cancer tissue was increased with the decrease of the degree of tumor differentiation (P<0.05), but was irrelevant to age, sex, tumor size, tumor infiltration depth and lymph node metastasis (all P>0.05). Conclusion: CHOP/GADD153 protein expression is increased in colon cancer tissue, and is closely related to degree of differentiation of colon cancer, which suggests that ERS may probably participate in the regulation of tumor differentiation.
Keywords：