文章摘要

乳腺癌患者外周血中T 细胞亚群与淋巴结转移和组织学分级的关系

作者: 1曾繁余, 2彭德珍, 1张珊, 1唐巍, 3张秋瑾, 3刘文慧, 3骆耐香
1 桂林医学院附属医院普外二科，广西 桂林 541001
2 桂林医学院附属医院检验科，广西 桂林 541001
3 桂林医学院 免疫学教研室， 广西 桂林 541004
通讯: 骆耐香 Email: luonaixiang@163.com
DOI: 10.3978/.10.3978/j.issn.1005-6947.2015.11.012
基金: 国家自然科学基金资助项目, 81260317 广西壮族自治区卫生厅基金资助项目, Z2013469 桂林市科学技术 局科技攻关基金资助项目, 20140120-1-10

摘要

目的：探讨乳腺癌患者外周血中T 细胞亚群的变化及其与淋巴结转移和组织学分级的关系。方法：用流式细胞术检测86 例乳腺癌患者以及20 例乳腺腺病患者外周血T 细胞亚群的百分率。结果：乳腺癌患者外周血总T 细胞与CD4+T 细胞百分数与腺病患者无统计学差异（均P>0.05），但CD8+T 细胞百分数低于腺病患者（P<0.05）。乳腺癌患者中，淋巴结转移者CD4+T 细胞百分数高于无淋巴结转移者（P<0.05）；CD8+T 细胞百分数随组织学分级增加而升高（P<0.05）。结论：乳腺癌患者存在细胞免疫功能紊乱，外周血中CD4+T 细胞、CD8+T 细胞比例的变化分别与淋巴结转移、组织学分级密切相关。监测外周血T 细胞亚群的变化，有助于病情及预后的判断。
关键词： 乳腺肿瘤 T 淋巴细胞亚群 淋巴转移 肿瘤分级

Relations of peripheral blood T cell subsets with lymph node metastasis and histological grade in breast cancer patients

Authors: 1ZENG Fanyu, 2PENG Dezhen, 1ZHANG Shan, 1TANG Wei, 3ZHANG Qiujin, 3LIU Wenhui, 3LUO Naixiang
1 The Second Department of General Surgery, Affiliated Hospital, Guilin Medical University, Guilin, Guangxi 541001, China
2 Department of Laboratory Medicine, Affiliated Hospital, Guilin Medical University, Guilin, Guangxi 541001, China
3 Department of Immunology, Guilin Medical University, Guilin, Guangxi 541004, China

CorrespondingAuthor:LUO Naixiang Email: luonaixiang@163.com

Abstract

Objective: To investigate the alterations of peripheral blood T cell subsets and their relations with lymph node metastasis and histological grade in breast cancer patients. Methods: The percentages of T cell subsets in peripheral blood in 86 patients with breast cancer and 20 patients with mammary adenosis were determined by flow cytometry. Results: There was no significant difference in percentages of peripheral blood total T cells and CD4+ T cells between breast cancer patients and mammary adenosis patients (both P>0.05), but the percentage of peripheral blood CD8+ T cells in breast cancer patients was lower than that of mammary adenosis patients (P<0.05). In breast cancer patients, the percentage of CD4+ T cells in cases with lymph node metastasis was higher than that of cases without lymph node metastasis (P<0.05); the percentage of CD8+ T cells was increased with histological grade increase (P<0.05). Conclusion: There are cell-mediated immune disorders in breast cancer patients, and the alteration in proportion of CD4+ T cells and CD8+ T cells in peripheral blood is closely related to lymph node metastasis and tumor histological grade, respectively. Monitoring of the alterations in peripheral blood T cell subsets may be helpful for estimation of the disease status and prognosis.
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