microRNA-25 高表达与直肠癌细胞的侵袭和迁移的关系
| 作者: |
1徐晓慧,
1孙欣,
1刘娜,
1矫健,
1李秋阳,
1刘京平,
1林方才
1 首都医科大学电力教学医院 普通外科，北京 100073 |
| 通讯: |
林方才
Email: fc-lin@126.com |
| DOI: | 10.3978/.10.3978/j.issn.1005-6947.2015.12.015 |
摘要
目的：探讨microRNA-25（miR-25）在直肠癌细胞中的表达及作用。 方法：检测多种不同直肠癌细胞中miR-25 的表达，并检测直肠癌细胞转染miR-25 前体（pre-miR-25） 上调miR-25 的表达或转染miR-25 抑制剂（anti-miR-25）下调miR-25 的表达后生物学行为的变化。 结果：与正常直肠黏膜组织比较，不同的直肠癌细胞中miR-25 的表达均不同程度的明显升高（均 P<0.05）。在miR-25 表达水平相对较低的直肠癌HR-834 细胞中转染pre-miR-25， 在miR-25 高表 达的直肠癌SW-837 细胞中转染anti-miR-25 后， 两种细胞的增殖、细胞周期、凋亡无明改变（ 均 P>0.05），但侵袭及迁移能力在HR-834 细胞的明显增强，SW-837 细胞减弱（均P<0.05）。 结论：miR-25 在直肠癌细胞中的表达升高，且其升高程度与细胞的侵袭和迁移能力密切相关。
关键词：
直肠肿瘤
微RNAs
肿瘤侵润
Relations of high microRNA-25 expression with migration and invasion of rectal carcinoma cells
CorrespondingAuthor:LIN Fangcai Email: fc-lin@126.com
Abstract
Objective: To investigate microRNA-25 (miR-25) expression in rectal carcinoma cells and its action. Methods: The miR-25 expressions in several different rectal cell lines were determined, and then the changes in biological behaviors in rectal cancer cells after up- or down-regulation of miR-25 expression with miR-25 precursor (pre-miR-25) or miR-25 inhibitor (anti-miR-25) transfection were examined. Results: Compared with normal rectal mucosal tissue, the miR-25 expressions in all tested rectal cancer cells were significantly increased with varying degrees (all P<0.05). In rectal cancer HR-834 cells with relatively low miR-25 expression after pre-miR-25 transfection and in rectal cancer SW-837 cells with high miR-25 expression after antimiR- 25 transfection, the proliferation, cell cycle and apoptosis showed no significant change (all P<0.05), but the invasion and migration abilities in HR-834 cells were increased, and SW-837 cells were decreased significantly (all P<0.05). Conclusion: The miR-25 expression is elevated in rectal cancer cells, and its degree of increase is closely related to the invasion and migration abilities of rectal cancer cells.
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