文章摘要

JAK2/STAT3 信号通路在肝细胞性肝癌中表达及意义

作者: 1赵冀安, 2刘文聪, 1孙会凤, 1周小慧, 1陈进军, 3贾聿明, 1朱俊青
1 河北医科大学第一医院肝胆外科，河北 石家庄 050013
2 河北医科大学第一医院超声科，河北 石家庄 050013
3 河北医科大学第四医院 肝胆外科，河北 石家庄 050011
通讯: 朱俊青 Email: 171955999@qq.com
DOI: 10.3978/.10.3978/j.issn.1005-6947.2016.01.013
基金: 河北省卫生和计划生育委员会基金资助项目, 20150634

摘要

目的：探讨JAK2/STAT3 信号通路在人肝细胞性肝癌（HCC）组织中的表达以及其意义。方法：用免疫组化法和Western blot 法检测75 例HCC 组织及其相应的癌旁组织JAK2 与STAT3 蛋白的表达，分析两者与HCC 患者病理特征及预后的关系。结果：JAK2 与STAT3 蛋白在HCC 组织中的阳性表达率及表达量均高于相应的癌旁组织（62.7% vs.5.3%，69.3% vs. 9.3%；均P<0.05）；JAK2 与STAT3 蛋白在HCC 组织中的表达呈明显正相关（r=0.383，P<0.01）。JAK2 和STAT3 蛋白的表达与肝硬化、门静脉癌栓、肿瘤分化程度、临床分期明显有关（均P<0.05）。生存分析显示，JAK2 与STAT3 蛋白高表达患者的生存率及生存期均明显低于各自的低表达患者（χ2=13.591；χ2=6.842，均P<0.05）；Cox 比例风险回归模型分析表明，JAK2 与STAT3 蛋白以及门静脉癌栓、肿瘤分化程度、临床分期均为影响HCC 预后的独立危险因素（均P<0.05）。结论：JAK2/STAT3 信号通路在HCC 组织中活性增高，且其活性高低与HCC 患者预后密切相关。
关键词： 癌，肝细胞 Janus 激酶2 STAT3 转录因子 预后

Expression of JAK2/STAT3 signaling pathway in human hepatocellular carcinoma and its significance

Authors: 1ZHAO Ji’an, 2LIU Wencong, 1SUN Huifeng, 1ZHOU Xiaohui, 1CHEN Jinjun, 3JIA Yuming, 1ZHU Junqing
1 Department of Hepatobiliary Surgery, the First Affiliated Hospital, Hebei Medical University, Shijiazhuang 050013, China
2 Department of Ultrasonography, the First Affiliated Hospital, Hebei Medical University, Shijiazhuang 050013, China
3 Department of Hepatobiliary Surgery, the Fourth Affiliated Hospital, Hebei Medical University, Shijiazhuang 050011, China

CorrespondingAuthor:ZHU Junqing Email: 171955999@qq.com

Abstract

Objective: To investigate the expression of JAK2/STAT3 signaling pathway in human hepatocellular carcinoma (HCC) tissue and its significance. Methods: The protein expressions of JAK2 and STAT3 in specimens of HCC tissues and matched adjacent noncancer tissues from 75 patients were detected by immunohistochemical staining and Western blot analysis. The relations of their expressions with the clinicopathologic features and prognosis of the patients were analyzed. Results: Both positive expression rate (62.7% vs. 5.3%; 69.3% vs. 9.3%) and expression level of either JAK2 or STAT3 protein in HCC tissue were significantly higher than those in the adjacent non-cancer tissue (all P<0.05); there was significant correlation between the expressions of JAK2 and STAT3 protein in HCC tissue (r=0.383, P<0.01). Both expressions of JAK2 and STAT3 were significantly related to cirrhosis, portal vein tumor thrombus, degree of tumor differentiation, and clinical stage (all P<0.05). Survival analysis showed that both survival rate and survival time in patients with high JAK2 or STAT3 expression were significantly decreased compared with corresponding low expression cases (χ2=13.591; χ2=6.842, both P<0.05). Cox proportional hazard regression model analysis indicated that the expression of JAK2 and STAT3 protein along with portal vein tumor thrombus, degree of tumor differentiation, clinical stage were independent risk factors for HCC (all P<0.05). Conclusion: The activity of the JAK2/STAT3 signaling pathway is increased in HCC tissue and the degree of its activation is closely related to the prognosis of HCC patients.
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