文章摘要

FK506结合蛋白5在肝细胞癌中的表达及其临床意义

作者: 1闫亭亭, 2龙晨, 2蔡文武, 2王继龙
1 中南大学湘雅公共卫生学院，湖南 长沙410008
2 中南大学湘雅二医院 普通外科，湖南 长沙410011
通讯: 王继龙 Email: wangjilong@csu.edu.cn
DOI: 10.3978/.10.3978/j.issn.1005-6947.2017.01.010
基金: 中南大学研究生自主探索创新项目资助, 2014zzts088

摘要

目的：探讨FK506结合蛋白5（FKBP5）在肝细胞癌（HCC）中的表达及临床意义。方法：用qRT-PCR和Western blot法检测30对新鲜HCC及相应癌旁组织中FKBP5 mRNA和蛋白的表达；免疫组化法检测107例HCC患者石蜡标本中FKBP5的表达，并分析其与HCC临床病理特征及预后的关系。结果：30例新鲜HCC标本中，26例（86.7%）HCC组织FKBP5 mRNA的表达水平明显高于相应癌旁组织，HCC组织中FKBP5蛋白表达量明显高于其癌旁组织。石蜡标本分析显示，FKBP5蛋白在HCC组织中的高表达率明显高于癌旁组织（63.6% vs. 28.0%，P<0.001）；FKBP5的表达水平与肿瘤大小（P=0.009）、肿瘤个数（P=0.018）、微血管侵犯（P=0.020）、TNM分期（P=0.009）和BCLC分期（P=0.024）有关，且FKBP5高表达患者术后总体生存率和无瘤生存率均明显低于低表达组（均P<0.001）；FKBP5高表达是HCC患者术后总体生存（HR=1.992，95% CI=1.127~3.522，P=0.018）和无瘤生存（HR=1.787，95% CI=1.067~2.992，P=0.027）的独立危险因素。结论：FKBP5在HCC组织中高表达，且与患者不良临床病理特征及预后密切相关，提示其是一个潜在的HCC预后分子标志物。
关键词： 癌，肝细胞 他克莫司结合蛋白质类 预后

Expression of FK506 binding protein 5 in hepatocellular carcinoma and its clinical significance

Authors: 1YAN Tingting, 2LONG Chen, 2CAI Wenwu, 2WANG Jilong
1 Xiangya School of Public Health, Central South University, Changsha 410008, China
2 Department of General Surgery, the Second xiangya Hospital, Central South University, Changsha 410011, China

CorrespondingAuthor:WANG Jilong Email: wangjilong@csu.edu.cn

Abstract

Objective: To investigate the expression of FK506 binding protein 5 (FKBP5) in hepatocellular carcinoma (HCC) and its clinical significance. Methods: The expressions of FKBP5 mRNA and protein in 30 fresh HCC and the adjacent tissue paired samples were determined by RT-PCR and Western blot, respectively. Then, immunohistochemical staining was performed in paraffin-embedded specimens from 107 HCC patients and the relations of FKBP5 expression with clinicopathologic features and prognosis of HCC patients were analyzed. Results: Of the 30 fresh HCC tissues, 26 samples (86.7%) showed that the FKBP5 mRNA expression level was markedly higher in HCC tissue than that in its adjacent tissue, and FKBP5 protein expression level in HCC tissue was also markedly increased compared with adjacent normal tissue. In the 107 paraffin-embedded specimens, the high expression rate of FKBP5 protein in HCC tissues was significantly higher than that in adjacent tissues (63.6% vs. 28.0%, P<0.001); high FKBP5 expression was significantly associated with tumor size (P=0.009), lesion number (P=0.018), microvascular invasion (P=0.020), TNM stage (P=0.009) and BCLC stage (P=0.024); both overall survival rate and disease-free survival rate in patients with high FKBP5 expression were significantly lower than those in patients with low FKBP5 expression (both P<0.001); high FKBP5 expression was an independent risk factor for either overall survival (HR=1.992, 95% CI=1.127–3.522, P=0.018) or disease-free survival (HR=1.787, 95% CI=1.067–2.992, P=0.027). Conclusion: FKBP5 expression is increased in HCC tissue, and its overexpression is associated with unfavorable clinicopathologic features and poor prognosis of HCC patients, suggesting that FKBP5 may be a potential prognostic indicator for HCC.
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