文章摘要

纳米金增加阿霉素耐药肝癌细胞株敏感性的实验研究

作者: 1邵明涛, 1,2潘运龙, 3覃莉, 1丁晖, 2赵晓旭, 1吴晓波
1 暨南大学附属第一医院 胃肠外科，广东 广州 510632
2 暨南大学附属第一医院 伽玛刀中心，广东 广州 510632
3 暨南大学医学院 组织胚胎学教研室，广东 广州 510632
通讯: 潘运龙 Email: tpanyl@jnu.edu.cn
DOI: 10.3978/.10.3978/j.issn.1005-6947.2015.01.010
基金: 国家基础研究973 计划资助项目, 2010CB833603 国家自然科学基金资助项目, 81472849 暨南大学第一临 床医学院科研培育基金资助项目, 511005004 暨南大学第一临床医学院开放基金资助项目, 511005026

摘要

目的：观察纳米金（GNP）人耐药肝癌细胞株耐药性的逆转作用。 方法：采用氯金酸柠檬酸三钠还原法制备并鉴定；分别用GNP 与阿霉素（ADM）组单独或联合作用于 ADM 耐药的肝癌细胞株HepG2/ADM，以未处理的HepG2/ADM 细胞为对照，用MTT 法检、流式细胞 术检测细胞的增殖与凋亡情况；紫外分光光度计检测HepG2/ADM 细胞经ADM 单独作用以及GNP 与 ADM 联合作用后细胞内ADM 浓度。 结果：与对照细胞比较，ADM 单独作用及GNP 与ADM 联合作用后，HepG2/ADM 的增殖均明显抑制、 凋亡率明显升高，但后者的作用明显强于前者（均P<0.05），而GNP 单独作用对细胞的增殖与凋亡无 明显影响（均P>0.05）；GNP+ADM 作用后，HepG2/ADM 细胞内的ADM 含量较ADM 单独作用后的 ADM 含量明显增加[（2.92±0.13）μg/L vs.（1.68±0.74）μg/L，P<0.05]。 结论：GNP 可增加HepG2/ADM 细胞对ADM 的敏感性，该作用可能与其增加HepG2/ADM 细胞内的 ADM 浓度有关。
关键词： 癌，肝细胞 金属纳米粒子 阿霉素 多药耐药

Gold nanoparticles re-sensitizing adriamycin-resistant hepatocellular carcinoma cells: an experimental study

Authors: 1SHAO Mingtao, 1,2PAN Yunlong, 3QIN Li, 1DING Hui, 2ZHAO Xiaoxu, 1WU Xiaobo
1 Department of Gastrointestinal Surgery, the First Affiliated Hospital, Jinan University, Guangzhou 510632, China
2 Gamma Knife Center, the First Affiliated Hospital, Jinan University, Guangzhou 510632, China
3 Department of Histology and Embryology, Jinan University, Guangzhou 510632, China

CorrespondingAuthor:PAN Yunlong Email: tpanyl@jnu.edu.cn

Abstract

Objective: To investigate the drug resistance reversing effect of gold nanoparticles (GNPs) on drug-resistant human hepatocellular carcinoma cell line. Methods: GNPs were synthesized by reducing hydrogen tetrachloroaurate using tri-sodium citrate, and identified. Adriamycin (ADM)-resistant hepatocellular carcinoma HepG2/ADM cells were treated with GNP and adriamycin (ADM), alone or in combination, using HepG2/ADM cells without any treatment as a control, and then the cell proliferation and apoptosis were determined by MTT assay and flow cytometry, respectively. The intracellular ADM concentration of HepG2/ADM cells after exposure to ADM alone or GNP plus ADM were determined by ultraviolet-visible spectrophotometer. Results: Compared with control cells, the cell proliferation was inhibited and apoptosis rate was increased significantly in HepG2/ADM cells treated with ADM along or combination with GNP, and these effects were more remarkable in the latter than in the former (all P<0.05). The intracellular ADM concentration in HepG2/ADM cells exposed to GNP plus ADM was significantly higher than in those exposed to ADM alone [(2.92±0.13) μg/L vs. (1.68±0.74) μg/L, P<0.05]. Conclusion: GNP can enhance the sensitivity of HepG2/ADM cells to ADM, and this effect may be related to its increasing the intracellular ADM accumulation in HepG2/ADM cells.
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