文章摘要

TGF-β1、Smad7 与树突状细胞在结直肠癌肝转移中作用的初步研究

作者: 1刘贤伟, 2曹俊达, 1熊功友, 1余昌达, 3张建华, 3董钦生, 3周丁华
1 江西省九江市第一人民医院 普通外科，江西 九江 332200
2 江西省九江市第一人民医院 心血管内科，江西 九江 332200
3 中国人民解放军第二炮兵总医院 普通 外科，北京 100088
通讯: 周丁华 Email: zhoudh@sina.com
DOI: 10.3978/.10.3978/j.issn.1005-6947.2015.01.012

摘要

目的：探讨结直肠癌肝转移（CRLM）组织中TGF-β1、Smad7 表达和树突状细胞（DC）的变化及临床意义。 方法：采用免疫组化法检测TGF-β1、Smad7、CD1α（未成熟DC 标志）和CD83（成熟DC 标志） 在CRLM 组织和CRLM 术后切缘阴性的正常肝组织中的表达，分析CRLM 组织中以上指标表达之间的 关系以及与原发灶特征的关系。 结果：CRLM 组织中TGF-β1、Smad7、和CD1α 的表达明显高于正常肝组织组（均P<0.05）， 且 CRLM 组织中彼此表达之间呈正相关关系（r=0.455、r=0.623、r=0.448，均P<0.05）；CD83 在CRLM 组织中基本为全阴性表达，而在正常肝组织组中仅2 例呈弱阳性表达。TGF-β1、Smad7、和CD1α 的表达均与CRLM 患者原发灶的肿瘤浸润深度、有无淋巴结转移有关（均P<0.05），而与原发灶肿瘤 分化程度无关（P>0.05）。 结论：TGF-β1、Smad7α 的高表达及未成熟DC 数量的增加可能共同促进CRLM 的发生、发展。
关键词： 肝肿瘤/ 继发性 结直肠肿瘤 肿瘤转移 树突状细胞 转化生长因子β1

Preliminary study of roles of TGF- 1, Smad7 and dendritic cells in colorectal cancer liver metastases

Authors: 1LIU Xianwei, 2CAO Junda, 1XIONG Gongyou, 1YU Changda, 3ZHANG Jianhua, 3DONG Qinsheng, 3ZHOU Dinghua
1 Department of General Surgery, Jiujiang First People’s Hospital, Jiujiang, Jiangxi 332000, China
2 Department of Cardiovascular Medicine, Jiujiang First People’s Hospital, Jiujiang, Jiangxi 332000, China
3 Department of General Surgery, the Second Artillery General Hospital of Chinese PLA, Bingjing 100088, China

CorrespondingAuthor:ZHOU Dinghua Email: zhoudh@sina.com

Abstract

Objective: To investigate the expressions of TGF-β1 and Smad7 and change of dendritic cells (DCs) in colorectal cancer liver metastases (CRLM) tissue and the clinical significance. Methods: The expressions of TGF-β1, Smad7, CD1α (marker of immature DCs) and CD83 (marker of mature DCs) in resection specimens of CRLM tissue and normal liver tissue around the CRLM with negative margin were determined by immunohistochemical staining. The mutual relations among these expressions in CRLM tissue and their relations with the characteristics of the primary tumor were analyzed. Results: The expressions of TGF-β1, Smad7, and CD1α in CRLM tissue were significantly higher than those in normal liver tissue (all P<0.05), and their expressions showed mutual positive correlations in CRLM tissue (r=0.455, r=0.623, r=0.448, all P<0.05); CD83 expression was negative in almost all the studied CRLM tissue specimens, and only two cases of weak positive expression were found in the normal liver tissue specimens. All expressions of TGF-β1, Smad7 and CD1α were significantly related to the depth of invasion of the primary tumor and lymph node metastasis (all P<0.05), but irrelevant to differentiation of the primary tumor (P>0.05) in CRLM patients. Conclusion: The high TGF-β1 and Smad7 expressions and increased immature DCs number may together promote the occurrence and development of CRLM.
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