脾切除对肝纤维化大鼠肝脏TGF-β1和α-SMA表达的影响
| 作者: |
1钱言,
1孙礼侠,
2吴鹏飞,
3顾鑫瑾
1 安徽省芜湖市第二人民医院 肝胆外科，安徽 芜湖 241000 2 安徽省芜湖市第一人民医院 普通外科，安徽 芜湖 241000 3 浙江省宁波市鄞州人民医院 普通外科，浙江 宁波 315040 |
| 通讯: |
孙礼侠
Email: slx64@126.com |
| DOI: | 10.3978/.10.3978/j.issn.1005-6947.2016.07.016 |
摘要
目的：通过观察肝纤维化模型大鼠不同时间行脾切除术后肝组织TGF-β1与α-SMA mRNA水平的变化，探讨脾切除对肝纤维化发展的影响。方法：50只雄性SD大鼠随机分为正常对照组、肝硬化模型组（模型组）、肝硬化模型+切脾组（切脾组），采用腹腔内注射40%四氯化碳诱导肝纤维化模型，切脾组大鼠分别于建模后早期（第2周）、中期（第4周）、晚期（第6周）分批行脾切除术。造模后第8周处死所有大鼠，HE染色观察肝脏组织的病理变化；qRT-PCR检测肝组织TGF-β1和α-SMA的mRNA的表达水平。结果：正常对照组肝小叶结构完整，模型组肝小叶结构被破坏，纤维组织明显增生，假小叶形成；各切脾组均有不同程度的纤维化病变，且随着切脾时间的延迟逐渐加重，但均轻于模型组。qRT-PCR结果显示，与正常对照组比较，其余各组肝组织TGF-β1和α-SMA的mRNA的水平均较明显升高（均P<0.05），升高程度均表现为：模型组>晚期切脾组>中期切脾组>早期切脾组（均P<0.05）。结论：脾切除能降低了肝组织TGF-β1与α-SMA的表达水平，故脾脏可能参与了肝纤维化的发生发展。
关键词：
肝硬化
脾切除术
转化生长因子β1
大鼠
Effect of splenectomy on TGF-β1 and α-SMA expressions in the liver of rats with hepatic fibrosis
CorrespondingAuthor:SUN Lixia Email: slx64@126.com
Abstract
Objective: To study the influence of splenectomy on development of hepatic fibrosis through observation of the changes in mRNA expressions of TGF-β1 and α-SMA in liver tissue after splenectomy performed at different time periods in rats with hepatic fibrosis.
Methods: Fifty male SD rats were randomly allocated into normal control group, liver fibrosis model group (model group) and liver fibrosis model with splenectomy group (splenectomy group). The liver fibrosis model was induced by intraperitoneal injection of 40% CCl4, and rats in splenectomy group underwent splenectomy at early stage (2 weeks), mid stage (4 weeks) and late stage (6 weeks) after model induction in batches, respectively. All rats were sacrificed at 8 weeks after model induction, the pathological changes in the liver tissues were evaluated by HE staining, and the mRNA expression levels of TGF-β1 and α-SMA in the liver tissues were determined by qRT-PCR method. Results: The structure of hepatic lobules was intact in normal control group, while in model group, it was destroyed with fibrous tissue hyperplasia and pseudo-lobule formation; pathological changes of hepatic fibrosis of different degrees were found in all splenectomy groups, which presented with increasing severity with the lag of time of splenectomy, but all were milder than that in model group. The results of qRT-PCR showed that compared with normal control group, the mRNA expression levels of both TGF-β1 and α-SMA in all other groups were significantly increased (all P<0.05), and the increasing degrees in both factors were presented as follows: model group > late-stage splenectomy group > mid-stage splenectomy group > early-stage splenectomy group (all P<0.05). Conclusion: Splenectomy can reduce the expression levels of TGF-β1 and α-SMA in liver tissue, which suggests that the spleen may be involved in the occurrence and developent of liver fibrosis.
Keywords：
Methods: Fifty male SD rats were randomly allocated into normal control group, liver fibrosis model group (model group) and liver fibrosis model with splenectomy group (splenectomy group). The liver fibrosis model was induced by intraperitoneal injection of 40% CCl4, and rats in splenectomy group underwent splenectomy at early stage (2 weeks), mid stage (4 weeks) and late stage (6 weeks) after model induction in batches, respectively. All rats were sacrificed at 8 weeks after model induction, the pathological changes in the liver tissues were evaluated by HE staining, and the mRNA expression levels of TGF-β1 and α-SMA in the liver tissues were determined by qRT-PCR method. Results: The structure of hepatic lobules was intact in normal control group, while in model group, it was destroyed with fibrous tissue hyperplasia and pseudo-lobule formation; pathological changes of hepatic fibrosis of different degrees were found in all splenectomy groups, which presented with increasing severity with the lag of time of splenectomy, but all were milder than that in model group. The results of qRT-PCR showed that compared with normal control group, the mRNA expression levels of both TGF-β1 and α-SMA in all other groups were significantly increased (all P<0.05), and the increasing degrees in both factors were presented as follows: model group > late-stage splenectomy group > mid-stage splenectomy group > early-stage splenectomy group (all P<0.05). Conclusion: Splenectomy can reduce the expression levels of TGF-β1 and α-SMA in liver tissue, which suggests that the spleen may be involved in the occurrence and developent of liver fibrosis.