文章摘要

EphA2与VEGF-C在胃癌中的表达及其与淋巴管生成的关系

作者: 1朱振华, 2袁伟杰, 2黄昌浩, 2陈子华
1 湖南省长沙市中心医院 普通外科，湖南 长沙 410004
2 中南大学湘雅医院 普通外科，湖南 长沙 410008
通讯:
DOI: 10.3978/.10.3978/j.issn.1005-6947.2017.04.012
基金: 高等学校博士学科点专项科研基金资助项目, 20130162110026

摘要

目的：探讨胃癌组织中EphA2与VEGF-C的表达及临床意义。方法：用免疫组化法检测82例胃癌组织及其癌旁组织中EphA2与VEGF-C蛋白的表达，以及淋巴管密度（LVD）；用real-time PCR检测20例胃癌组织及其癌旁组织中EphA2与VEGF-C mRNA的表达。分析EphA2与VEGF-C表达与患者临床病理特点及淋巴管生成的关系。结果：EphA2与VEGF-C在胃癌组织中的阳性表达率均明显高于癌旁正常组织（65.8% vs. 42.6%；71.9% vs. 39.0%，均P<0.05）。EphA2与VEGF-C的高表达与肿瘤浸润深度、淋巴结转移、TNM分期有关（均P<0.001）。EphA2蛋白与VEGF-C蛋白高表达的胃癌组织中LVD计数分别明显高于两者低表达胃癌组织（15.25±5.41 vs. 10.95±5.41，P=0.001；14.87±5.71 vs. 11.00±5.01，P=0.006）。胃癌组织中EphA2与VEGF-C蛋白以及mRNA表达均呈正相关（r=0.375，P=0.001；r=0.559，P=0.01）。结论：EphA2与VEGF-C在胃癌组织中均呈高表达，且可能共同促进淋巴管生成与胃癌淋巴结转移。
关键词： 胃肿瘤 受体，Eph家族 血管内皮生长因子C 淋巴管生成

Expressions of EphA2 and VEGF-C in gastric cancer and their association with lymphangiogenesis

Authors: 1ZHU Zhenhua, 2YUAN Weijie, 2HUANG Changhao, 2CHEN Zihua
1 Department of General Surgery, Changsha Central Hospital, Changsha 410004, China
2 Department of General Surgery, Xiangya Hospital, Central South University, Changsha 410008, China

Abstract

Objective: To investigate the EphA2 and VEGF-C expressions in gastric cancer and their clinical significance. Methods: The protein expressions of VEGF-C and EphA2 as well as the lymphatic vessel density (LVD) in 82 paired specimens of gastric cancer and adjacent gastric tissue were determined by immunohistochemical staining. The mRNA expressions of EphA2 and VEGF-C in 20 paired specimens of gastric cancer and adjacent gastric tissue were detected by real-time RT-PCR. The relations of EphA2 and VEGF-C expressions with the clinicopathologic characteristics of the patients and lymphangiogenesis were analyzed. Results: The positive expression rates of both EphA2 and VEGF-C in gastric cancer tissue were significantly higher than those in adjacent gastric tissue (65.8% vs. 42.6%; 71.9% vs. 39.0%, both P<0.05). The high expressions of EphA2 and VEGF-C were significantly associated with the depth of tumor invasion, lymph node metastasis and TNM stage (all P<0.001). The LVD count in gastric cancer tissue with high EphA2 or VEGF-C expression was significantly higher than that in gastric cancer tissue with low EphA2 or VEGF-C expression (15.25±5.41 vs. 10.95±5.41, P=0.001; 14.87±5.71 vs. 11.00±5.01, P=0.006). There was a significant correlation between EphA2 and VEGF-C in either protein or mRNA expression in gastric cancer tissue (r=0.375, P=0.001; r=0.559, P=0.01). Conclusion: Both EphA2 and VEGF-C expressions are increased in gastric cancer tissue, and they may jointly promote lymphangiogenesis and lymph node metastasis in gastric cancer.
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