Objective: To investigate the miR-567 expression in pancreatic cancer cells and its function. Methods: The miR-567 expressions in normal pancreatic duct epithelial cell line HPDE6-C7 and pancreatic carcinoma cell line Panc-1, AsPC-1, HPAC and BxPC-3 were determined by qRT-PCR. The miR-567 overexpression lentivirus vectors were transfected into the Panc-1 cells, and then, the changes in proliferation, apoptosis and migration ability as well as expressions of KPN4 mRNA and protein and apoptosis-associated proteins were analyzed by CCK-8 assay, flow cytometry, scratch wound healing assay, qRT-PCR and Western blot, respectively. Results: The miR-567 expressions in all the pancreatic cancer cell lines were significantly lower than that in normal pancreatic duct epithelial HPDE6-C7 cells (all P<0.05). In Panc-1 cells after transfection with miR-567 overexpression lentivirus vectors, the proliferation was decreased, apoptosis rate was increased, scratch healing rate was reduced, and both KPNA4 mRNA and protein expressions were down-regulated, while the caspase-3 and Bax protein expressions were up-regulated significantly (all P<0.05). Conclusion: MiR-567 expression is decreased in pancreatic cancer cells, and up-regulating its expression can inhibit the growth and migration ability of pancreatic cancer cells, and the mechanism may possibly be associated with its decreasing KPNA4 expression and increasing the expressions of apoptosis-associated proteins.