TUSC3基因在肝细胞癌中的表达及其临床意义
| 作者: |
1盛须仁,
1邢松歌,
1葛勇胜,
1荚卫东
1 中国科学技术大学附属第一医院（安徽省立医院） 肝脏外科/肝胆胰外科安徽省重点实验室，安徽 合肥 230001 |
| 通讯: |
荚卫东
Email: jwd1968@sina.com |
| DOI: | 10.3978/.2018.01.012 |
| 基金: | 安徽省科学技术厅科技计划基金资助项目（1704a0802150）。 |
摘要
目的：探讨TUSC3基因在肝细胞癌（HCC）中的表达及临床意义。
方法：收集92例行肝切除术且术后病理证实为HCC患者的石蜡组织标本及其临床病理资料，用免疫组化法检测TUSC3在HCC组织及相对应癌旁组织中的表达，分析TUSC3的表达情况与HCC患者临床病理因素的关系；用qRT-PCR及Western blot方法检测20对冷冻HCC及癌旁组织中TUSC3 mRNA与蛋白的表达。
结果：92例石蜡组织标本的免疫组化结果显示，56例（60.9%）HCC组织中TUSC3表达下调，癌旁组织中33例（35.9%）TUSC3表达下调，差异有统计学意义（P<0.001）；TUSC3在HCC组织中的低表达与肿瘤Edmondson分级（P=0.008）、TNM分期（P=0.031）、肿瘤直径有关（P=0.020）。20对新鲜标本的qRT-PCR与Western blot检测结果显示，肝癌组织中TUSC3的mRNA[（0.99±1.46） vs.（1.96±2.18）]与蛋白[（0.49±0.35 ）vs. （1.04±0.43）]相对表达量均明显低于癌旁组织（均P<0.05）。
结论：TUSC3基因在HCC中的表达下调，其低表达可能与HCC的恶性进程密切相关。
关键词：
癌，肝细胞；基因，肿瘤抑制；肿瘤侵润
方法：收集92例行肝切除术且术后病理证实为HCC患者的石蜡组织标本及其临床病理资料，用免疫组化法检测TUSC3在HCC组织及相对应癌旁组织中的表达，分析TUSC3的表达情况与HCC患者临床病理因素的关系；用qRT-PCR及Western blot方法检测20对冷冻HCC及癌旁组织中TUSC3 mRNA与蛋白的表达。
结果：92例石蜡组织标本的免疫组化结果显示，56例（60.9%）HCC组织中TUSC3表达下调，癌旁组织中33例（35.9%）TUSC3表达下调，差异有统计学意义（P<0.001）；TUSC3在HCC组织中的低表达与肿瘤Edmondson分级（P=0.008）、TNM分期（P=0.031）、肿瘤直径有关（P=0.020）。20对新鲜标本的qRT-PCR与Western blot检测结果显示，肝癌组织中TUSC3的mRNA[（0.99±1.46） vs.（1.96±2.18）]与蛋白[（0.49±0.35 ）vs. （1.04±0.43）]相对表达量均明显低于癌旁组织（均P<0.05）。
结论：TUSC3基因在HCC中的表达下调，其低表达可能与HCC的恶性进程密切相关。
TUSC3 expression in hepatocellular carcinoma and its clinical significance
CorrespondingAuthor:JIA Weidong Email: jwd1968@sina.com
Abstract
Objective: To investigate the TUSC3 gene expression in hepatocellular carcinoma (HCC) and its clinical significance.
Methods: The paraffin-embedded tissue specimens and clinicopathologic data of 92 patients with HCC confirmed by postoperative pathology were collected, and the TUSC3 expressions in HCC and the adjacent tissues were detected by immunohistochemical staining and the relations of TUSC3 expression with clinicopathologic factors of the patients were analyzed. The mRNA and protein expressions of TUSC3 in 20 paired frozen tissue samples of HCC and adjacent tissues were determined by qRT-PCR and Western blot analysis, respectively.
Results: Results of immunohistochemical staining for the 92 paraffin-embedded tissue specimens showed TUSC3 expression down-regulated in 56 cases (60.9%) in HCC tissues, and only in 33 cases (35.9%) in adjacent tissues, and the difference was statistically significant (P<0.001); low TUSC3 expression was significantly related to Edmondson grade (P=0.008), TNM stage (P=0.031) and tumor size (P=0.020). Results of qRT-PCR and Western blot analysis of the 20 paired fresh samples showed that the relative expression levels of both TUSC3 mRNA [(0.99±1.46) vs. (1.96±2.18)] and protein [(0.49±0.35) vs. (1.04±0.43)] in HCC tissue were significantly lower than those in adjacent tissue (both P<0.05).
Conclusion: The TUSC3 gene expression is down-regulated in HCC tissue and the low TUSC3 expression may be closely related to the malignant process of HCC.
Keywords：
Carcinoma
Hepatocellular; Genes
Tumor Suppressor; Neoplasm Invasiveness
Methods: The paraffin-embedded tissue specimens and clinicopathologic data of 92 patients with HCC confirmed by postoperative pathology were collected, and the TUSC3 expressions in HCC and the adjacent tissues were detected by immunohistochemical staining and the relations of TUSC3 expression with clinicopathologic factors of the patients were analyzed. The mRNA and protein expressions of TUSC3 in 20 paired frozen tissue samples of HCC and adjacent tissues were determined by qRT-PCR and Western blot analysis, respectively.
Results: Results of immunohistochemical staining for the 92 paraffin-embedded tissue specimens showed TUSC3 expression down-regulated in 56 cases (60.9%) in HCC tissues, and only in 33 cases (35.9%) in adjacent tissues, and the difference was statistically significant (P<0.001); low TUSC3 expression was significantly related to Edmondson grade (P=0.008), TNM stage (P=0.031) and tumor size (P=0.020). Results of qRT-PCR and Western blot analysis of the 20 paired fresh samples showed that the relative expression levels of both TUSC3 mRNA [(0.99±1.46) vs. (1.96±2.18)] and protein [(0.49±0.35) vs. (1.04±0.43)] in HCC tissue were significantly lower than those in adjacent tissue (both P<0.05).
Conclusion: The TUSC3 gene expression is down-regulated in HCC tissue and the low TUSC3 expression may be closely related to the malignant process of HCC.