Oct-4、SOX2和KPNA2在三阴性乳腺癌中的表达及其临床意义
| 作者: |
1赵士锋,
1王数,
1冯林,
1李蔚然,
1姚佳奇,
1陶雅军
1 大连大学医学院 病理学教研室，辽宁 大连 116622 |
| 通讯: |
陶雅军
Email: sabrinatao@sina.com |
| DOI: | 10.3978/.2018.05.009 |
| 基金: | 大连大学大学生创新创业训练计划项目（2018188） |
摘要
目的：探讨三阴性乳腺癌（TNBC）组织中Oct-4、SOX2和KPNA2的表达及其临床意义。
方法：采用免疫组化方法检测Oct-4、SOX2和KPNA2在30例TNBC和30例非TNBC患者癌组织中的表达，分析三者的表达与TNBC患者临床病理因素及预后的关系。
结果：与非TNBC组织比较， TNBC组织中Oct-4的阳性表达率（86.7% vs. 73.3%，P<0.05）和SOX2的阳性表达率（90.0% vs. 70.0%，P<0.05）均明显升高，KPNA2的阳性表达率无统计学差异（P>0.05）。在TNBC组织中，Oct-4、SOX2和KPNA2彼此间的表达均呈正相关（Oct-4和SOX2：r=0.680，P<0.0001；Oct-4和KPNA2：r=0.581，P=0.0008；SOX2和KPNA2：r=0.770，P<0.0001）。在TNBC患者中，Oct-4的表达与年龄、组织学分级、淋巴结转移和TNM分期均有关，SOX2的表达与淋巴结转移和TNM分期有关，KPNA2的表达与淋巴结转移有关（均P<0.05）；Oct-4、SOX2、KPNA2阳性表达患者生存率明显低于各自阴性表达患者（均P<0.01）。
结论：Oct-4、SOX2在TNBC组织中表达均增加，且两者可能与KPNA2共同参与了TNBC的恶性进展。
关键词：
三阴性乳腺癌；八聚体转录因子类；SOX转录因子类；核胞浆转运蛋白类
方法：采用免疫组化方法检测Oct-4、SOX2和KPNA2在30例TNBC和30例非TNBC患者癌组织中的表达，分析三者的表达与TNBC患者临床病理因素及预后的关系。
结果：与非TNBC组织比较， TNBC组织中Oct-4的阳性表达率（86.7% vs. 73.3%，P<0.05）和SOX2的阳性表达率（90.0% vs. 70.0%，P<0.05）均明显升高，KPNA2的阳性表达率无统计学差异（P>0.05）。在TNBC组织中，Oct-4、SOX2和KPNA2彼此间的表达均呈正相关（Oct-4和SOX2：r=0.680，P<0.0001；Oct-4和KPNA2：r=0.581，P=0.0008；SOX2和KPNA2：r=0.770，P<0.0001）。在TNBC患者中，Oct-4的表达与年龄、组织学分级、淋巴结转移和TNM分期均有关，SOX2的表达与淋巴结转移和TNM分期有关，KPNA2的表达与淋巴结转移有关（均P<0.05）；Oct-4、SOX2、KPNA2阳性表达患者生存率明显低于各自阴性表达患者（均P<0.01）。
结论：Oct-4、SOX2在TNBC组织中表达均增加，且两者可能与KPNA2共同参与了TNBC的恶性进展。
Expressions of Oct-4, SOX2 and KPNA2 in triple-negative breast cancer and their clinical significance
CorrespondingAuthor:TAO Yajun Email: sabrinatao@sina.com
Abstract
Objective: To investigate the expressions of Oct-4, SOX2 and KPNA2 in triple-negative breast cancer (TNBC) and their clinical significance.
Methods: The expressions of Oct-4, SOX2 and KPNA2 in the cancer tissues from 30 TNBC patients and 30 non-TNBC patients were determined by immunohistochemical staining. The relations of their expressions with clinicopathologic factors and prognosis of the TNBC patients were analyzed.
Results: In TNBC tissue compared with non-TNBC tissue, the positive expression rates of Oct-4 protein (86.7% vs. 73.3%, P<0.05) and SOX2 protein (90.0% vs. 70.0%, P<0.05) were significantly increased, while the positive expression rate KPNA2 protein showed no significant difference (P>0.05). The expressions of Oct-4, SOX2 and KPNA2 were positively correlated to each other in TNBC tissue (Oct-4 and SOX2: r=0.680, P<0.0001; Oct-4 and KPNA2: r=0.581, P=0.0008; SOX2 and KPNA2: r=0.770, P<0.0001). In TNBC patients, the Oct-4 expression was significantly related to age, histological grade, lymph node metastasis and TNM stage, the SOX2 expression was significantly related to lymph node metastasis and TNM stage, and the KPNA2 expression was significantly related to lymph node metastasis (all P<0.05); the overall survival rate in cases with positive Oct-4, SOX2 or KPNA2 expression was significantly lower than that in those with corresponding negative expression (all P<0.01).
Conclusion: The expressions of Oct-4 and SOX2 are increased in TNBC tissue, and they may jointly participate with KPNA2 in the malignant progression of TNBC.
Keywords：
Triple Negative Breast Neoplasms; Octamer Transcription Factors; SOX Transcription Factors; Karyopherins
Methods: The expressions of Oct-4, SOX2 and KPNA2 in the cancer tissues from 30 TNBC patients and 30 non-TNBC patients were determined by immunohistochemical staining. The relations of their expressions with clinicopathologic factors and prognosis of the TNBC patients were analyzed.
Results: In TNBC tissue compared with non-TNBC tissue, the positive expression rates of Oct-4 protein (86.7% vs. 73.3%, P<0.05) and SOX2 protein (90.0% vs. 70.0%, P<0.05) were significantly increased, while the positive expression rate KPNA2 protein showed no significant difference (P>0.05). The expressions of Oct-4, SOX2 and KPNA2 were positively correlated to each other in TNBC tissue (Oct-4 and SOX2: r=0.680, P<0.0001; Oct-4 and KPNA2: r=0.581, P=0.0008; SOX2 and KPNA2: r=0.770, P<0.0001). In TNBC patients, the Oct-4 expression was significantly related to age, histological grade, lymph node metastasis and TNM stage, the SOX2 expression was significantly related to lymph node metastasis and TNM stage, and the KPNA2 expression was significantly related to lymph node metastasis (all P<0.05); the overall survival rate in cases with positive Oct-4, SOX2 or KPNA2 expression was significantly lower than that in those with corresponding negative expression (all P<0.01).
Conclusion: The expressions of Oct-4 and SOX2 are increased in TNBC tissue, and they may jointly participate with KPNA2 in the malignant progression of TNBC.