不同分子标记物在甲状腺乳头状癌中的表达改变及其诊断价值
| 作者: |
1汤文丽,
1高庆军,
2赵代伟
1 贵州医科大学附属医院 甲状腺外科，贵州 贵阳 550004 2 贵州医科大学第二附属医院 甲状腺外科，贵州 凯里 556000 |
| 通讯: |
赵代伟
Email: zhaodw@hotmail.com |
| DOI: | 10.3978/.2018.05.007 |
摘要
目的：探讨各种分子标记物在甲状腺乳头状癌（PTC）中表达变化及其对PTC的诊断价值。
方法：用免疫组化法检测85例PTC及115例良性甲状腺结节（BTN）患者的术后病理标本中TPO、CK19、Galectin-3、HBME-1及Ki-67的表达，用受试者工作特征曲线（ROC）及二元Logistic回归方程评估各标记物及其联合检测对PTC的诊断价值。
结果：与BTN组织比较，PTC组织中CK19、Galectin-3、HBME-1、Ki-67的表达水平均明显升高，而TPO的表达水平明显降低（均P<0.05）。单独检测时，TPO的ROC曲线下面积（AUC）值最大，为0.943；联合检测时，5种分子标记物联合检测的AUC值最大，为0.996，但与其他任何包含Galectin-3与TPO两种标记物的组合比较，差异均无统计学意义（均P>0.05）。
结论：多种分子标记物在PTC中的表达有改变，其中Galectin-3及TPO联合检测可能对提高PTC的诊断有较高的价值。
关键词：
甲状腺肿瘤；癌，乳头状；生物标记，肿瘤；诊断，鉴别
方法：用免疫组化法检测85例PTC及115例良性甲状腺结节（BTN）患者的术后病理标本中TPO、CK19、Galectin-3、HBME-1及Ki-67的表达，用受试者工作特征曲线（ROC）及二元Logistic回归方程评估各标记物及其联合检测对PTC的诊断价值。
结果：与BTN组织比较，PTC组织中CK19、Galectin-3、HBME-1、Ki-67的表达水平均明显升高，而TPO的表达水平明显降低（均P<0.05）。单独检测时，TPO的ROC曲线下面积（AUC）值最大，为0.943；联合检测时，5种分子标记物联合检测的AUC值最大，为0.996，但与其他任何包含Galectin-3与TPO两种标记物的组合比较，差异均无统计学意义（均P>0.05）。
结论：多种分子标记物在PTC中的表达有改变，其中Galectin-3及TPO联合检测可能对提高PTC的诊断有较高的价值。
Changes in expressions of different molecular markers and their diagnostic values in thyroid papillary carcinoma
CorrespondingAuthor:ZHAO Daiwei Email: zhaodw@hotmail.com
Abstract
Objective: To investigate the changes in expressions of a variety of molecular markers in thyroid papillary carcinoma (PTC) and their diagnostic values for PTC.
Methods: The expressions of TPO, CK19, Galectin-3, HBME-1 and Ki-67 in the postoperative pathological specimens from 85 PTC patients and 115 patients with benign thyroid nodules (BTN) were determined by immunohistochemical staining. The diagnostic values of each molecular marker and their combination for PTC were evaluated by using receiver operating characteristic curve (ROC) and binary Logistic regression equation.
Results: In PTC tissue compared with BTN tissue, the expression levels of CK19, Galectin-3, HBME-1, and Ki-67 were significantly increased, while the expression level of TPO was significantly decreased (all P<0.05). In single detection of each molecular marker, TPO showed the biggest value of the area under the ROC curve (AUC), which was 0.943; in combined detection, the combination detection of all the 5 molecular markers possessed the biggest AUC value of 0.996, but it showed no significant differences compared with any combinations that included both Galectin-3 and TPO (all P>0.05).
Conclusion: The expressions of multiple molecular markers are changed in PTC, and among them, the combination detection of Galectin-3 and TPO may have a relatively high diagnostic value for PTC.
Keywords：
Thyroid Neoplasms; Carcinoma
Papillary; Biomarkers
Tumor; Diagnosis
Differential
Methods: The expressions of TPO, CK19, Galectin-3, HBME-1 and Ki-67 in the postoperative pathological specimens from 85 PTC patients and 115 patients with benign thyroid nodules (BTN) were determined by immunohistochemical staining. The diagnostic values of each molecular marker and their combination for PTC were evaluated by using receiver operating characteristic curve (ROC) and binary Logistic regression equation.
Results: In PTC tissue compared with BTN tissue, the expression levels of CK19, Galectin-3, HBME-1, and Ki-67 were significantly increased, while the expression level of TPO was significantly decreased (all P<0.05). In single detection of each molecular marker, TPO showed the biggest value of the area under the ROC curve (AUC), which was 0.943; in combined detection, the combination detection of all the 5 molecular markers possessed the biggest AUC value of 0.996, but it showed no significant differences compared with any combinations that included both Galectin-3 and TPO (all P>0.05).
Conclusion: The expressions of multiple molecular markers are changed in PTC, and among them, the combination detection of Galectin-3 and TPO may have a relatively high diagnostic value for PTC.