大鼠门静脉系统血栓形成模型的建立和观察
| 作者: |
1张津瑜,
2陈文显,
1沈华,
1王雁,
1张国雷,
1慎华平,
1吴万波,
1魏云海
1 湖州市中心医院 普通外科，浙江 湖州 313000 2 湖州市中心医院 超声科，浙江 湖州 313000 |
| 通讯: |
魏云海
Email: wyhsj1008@163.com |
| DOI: | 10.3978/.2018.06.011 |
| 基金: | 浙江省科学技术厅科技计划项目（2014C33137）。 |
摘要
目的：评价间断结扎阻断门静脉的方法建立大鼠门静脉系统血栓（PVT）形成模型的可行性。
方法：将 90 只 SD 大鼠随机均分为实验组和对照组，实验组采用间断结扎阻断门静脉的方法诱导PVT，对照组仅开腹后行门静脉系统的游离。于术前、术后 30 min 以及术后 3、6、12、24 h 用血管超声多普勒观察两组 PVT 形成情况，测定两组大鼠静脉血 P- 选择素、D- 二聚体及纤维蛋白原含量，并于 24 h 后处死所有大鼠，肉眼观察两组大鼠 PVT 形成情况同时行病理学检查。
结果：术后实验组大鼠死亡 5 只，未形成血栓大鼠 6 只，形成稳定的 PVT 大鼠 34 只（75.56%）；对照组大鼠死亡 2 只，形成少量附壁血栓大鼠 3 只，未形成血栓大鼠 40 只，两组 PVT 形成率差异有统计学意义（P<0.05）。实验组大鼠血栓长度随时间推移逐渐增大。两组大鼠血栓形成相关指标在术前、术后 30 min 及术后 3 h 差异无统计学意义（均 P>0.05），但在术后 6 h 后各时间点实验组均明显高于对照组（均 P<0.05）。
结论：采用间断结扎阻断门静脉的方法可以建立稳定、符合临床实际的 PVT 大鼠模型，P- 选择素、D- 二聚体及纤维蛋白原含量可作为参考指标。
关键词：
血栓形成；门静脉；大鼠；模型，动物
方法：将 90 只 SD 大鼠随机均分为实验组和对照组，实验组采用间断结扎阻断门静脉的方法诱导PVT，对照组仅开腹后行门静脉系统的游离。于术前、术后 30 min 以及术后 3、6、12、24 h 用血管超声多普勒观察两组 PVT 形成情况，测定两组大鼠静脉血 P- 选择素、D- 二聚体及纤维蛋白原含量，并于 24 h 后处死所有大鼠，肉眼观察两组大鼠 PVT 形成情况同时行病理学检查。
结果：术后实验组大鼠死亡 5 只，未形成血栓大鼠 6 只，形成稳定的 PVT 大鼠 34 只（75.56%）；对照组大鼠死亡 2 只，形成少量附壁血栓大鼠 3 只，未形成血栓大鼠 40 只，两组 PVT 形成率差异有统计学意义（P<0.05）。实验组大鼠血栓长度随时间推移逐渐增大。两组大鼠血栓形成相关指标在术前、术后 30 min 及术后 3 h 差异无统计学意义（均 P>0.05），但在术后 6 h 后各时间点实验组均明显高于对照组（均 P<0.05）。
结论：采用间断结扎阻断门静脉的方法可以建立稳定、符合临床实际的 PVT 大鼠模型，P- 选择素、D- 二聚体及纤维蛋白原含量可作为参考指标。
Establishment and observation of portal vein thrombosis model in rats
CorrespondingAuthor:WEI Yunhai Email: wyhsj1008@163.com
Abstract
Objective: To evaluate the feasibility of establishing portal vein thrombosis (PVT) in rats by repeated interruption of the portal vein.
Methods: Ninety SD rats were randomly and equally divided into experimental group and control group. Rats in experimental group underwent repeated ligation of the portal vein for induction of PVT, and those in control group underwent exposure of the portal vein after laparotomy. At preoperative and postoperative 30 min as well as postoperative 3, 6, 12 and 24 h, the PVT formation in the two groups of rats was observed by vascular Doppler ultrasound, and the levels of P-selectin, D-dimer and fibrinogen in the venous blood of the rats were determined. All rats were sacrificed 24 h after operation, and PVT formation was observed by gross examination and pathological examination was also performed.
Results: In experimental group, 5 rats died, 6 rats did not have PVT formation and 34 rats (75.56%) developed stable PVT at 24 h after operation; in control group, 2 rats died, a small amount of mural thrombus was seen in 3 rats and no thrombosis was observed in the other 40 rats, and the difference in PVT formation had statistical significance between the two groups (P<0.05). The length of the thrombus in rats of experimental group was gradually increased with time prolongation. The variables associated with thrombosis showed no significant difference between the two groups at preoperative 30 min and postoperative 30 min and 3 h (all P>0.05), but they were significantly higher in experimental group than those in control group at each time point from postoperative 6 h (all P<0.05).
Conclusion: Repeated interruption of the portal vein can generate a stable and clinical relevant PVT model in rats. P-selectin level, D-dimer content and fibrinogen content can be used as reference indexes.
Keywords：
Thrombosis; Portal Vein; Rat; Models
Animal
Methods: Ninety SD rats were randomly and equally divided into experimental group and control group. Rats in experimental group underwent repeated ligation of the portal vein for induction of PVT, and those in control group underwent exposure of the portal vein after laparotomy. At preoperative and postoperative 30 min as well as postoperative 3, 6, 12 and 24 h, the PVT formation in the two groups of rats was observed by vascular Doppler ultrasound, and the levels of P-selectin, D-dimer and fibrinogen in the venous blood of the rats were determined. All rats were sacrificed 24 h after operation, and PVT formation was observed by gross examination and pathological examination was also performed.
Results: In experimental group, 5 rats died, 6 rats did not have PVT formation and 34 rats (75.56%) developed stable PVT at 24 h after operation; in control group, 2 rats died, a small amount of mural thrombus was seen in 3 rats and no thrombosis was observed in the other 40 rats, and the difference in PVT formation had statistical significance between the two groups (P<0.05). The length of the thrombus in rats of experimental group was gradually increased with time prolongation. The variables associated with thrombosis showed no significant difference between the two groups at preoperative 30 min and postoperative 30 min and 3 h (all P>0.05), but they were significantly higher in experimental group than those in control group at each time point from postoperative 6 h (all P<0.05).
Conclusion: Repeated interruption of the portal vein can generate a stable and clinical relevant PVT model in rats. P-selectin level, D-dimer content and fibrinogen content can be used as reference indexes.