文章摘要

上调miR-101 对肝细胞癌生物学行为的影响

作者: 1周密, 2汤成泳, 1张国庆, 1谭婧宇, 1吴忠均
1 重庆医科大学附属第一医院肝胆外科，重庆 400016
2 重庆医科大学附属第一医院药剂科，重庆 400016
通讯: 吴忠均 Email: wzjtcy@126.com
DOI: 10.3978/.10.3978/j.issn.1005-6947.2015.07.009

摘要

目的：探讨miR-101 在肝细胞癌（HCC）细胞生物学行为的影响。方法：分别将miR-101 模拟物或miRNA 阴性对照序列转染HepG2 和SMMC-7721 两种HCC 细胞，以无处理自然生长的两种细胞为各自空白对照，观察miR-101 对两种细胞增殖、集落形成能力、凋亡及周期的影响，以及对侵袭和迁移能力的影响。结果：与各自的空白对照细胞比较，转染miR-101 模拟物后的HepG2 和SMMC-7721 细胞的增值能力明显降低、细胞集落的形成明显减少、G0/G1 期的细胞比例升高、细胞凋亡率明显增加、迁移与侵袭细胞数明显减少（均P<0.05）；转染miRNA 阴性对照序列对两种细胞的以上指标无明显影响（均P>0.05）。结论：miR-101 可能在HCC 细胞中起到了抑癌基因的作用，上调其表达能抑制HCC 的恶性生物学行为。
关键词： 癌，肝细胞 微RNAs 肿瘤侵润

Impact of upregulating miR-101 on biological behavior of hepatocellular carcinoma

Authors: 1ZHOU Mi, 2TANG Chengyong, 1ZHANG Guoqing, 1TAN Jingyu, 1WU Zhongjun
1 Department of Hepatobiliary Surgery, the First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China
2 Department of Pharmacy, the First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China

CorrespondingAuthor:WU Zhongjun Email: wzjtcy@126.com

Abstract

Objective: To investigate the influence of miR-101 on the biological behavior of hepatocellular carcinoma (HCC) cells. Methods: The HCC HepG2 and SMMC-7721 cells were transfected with miR-101 mimics or miRNA negative control sequences, using the two types of cells cultured without any treatment as corresponding blank control, and then the effect of miR-101 on proliferation, colony formation ability, apoptosis and cell cycle, as well as the migration and invasion ability of the two types of cells were observed. Results: Compared with corresponding blank control, in either HepG2 or SMMC-7721 cells after transfection with miR-101 minics, the proliferative ability was decreased, colony formation was reduced, G0/G1 phase cell ratio was increased, apoptosis rate was increased, and the ability of migration and invasion was reduced markedly (all P<0.05); transfection of miRNA negative control sequences exerted no obvious effect on above parameters for the two types of cells (all P>0.05). Conclusion: miR-101 possibly plays a role as cancer suppressor gene in HCC cells, and upregulating its expression may suppress the malignant activity of HCC.
Keywords：