Objective: To investigate the feasibility of using laparoscopic minimally invasive technique to create animal model of biliary occlusion. Methods: Six mongrel dogs underwent laparoscopic common bile duct (CBD) ligation. The operative and postoperative variables were recorded, the preoperative and postoperative (day 3, 7 and 10) levels of white blood cells, liver function indexes, C-reactive protein (C-RP) and procalcitonin (PCT) were determined, and the CBD dilation and liver pathological changes were observed on postoperative day 10. Results: The overall operative time, time to recovery from anesthesia, time to first food intake and defecation was (39.17±3.4) min, (59.17±12.8) min, (3.5±0.63) h and 1 d, respectively. The WBC level was significantly elevated at postoperative day 3 (P<0.05), but soon recovered (P>0.05); the postoperative C-RP and PCT levels remained normal (all P>0.05); the postoperative transaminase levels were initially increased and then decreased slowly, but all were significantly higher than those of preoperative levels (all P<0.05); the postoperative bilirubin levels showed continuous increase (all P<0.05). Obvious CBD dilation was seen macroscopically and pathological examination showed marked dilation of the bile capillaries. Conclusion: Establishment of canine model of biliary occlusion using laparoscopic technique is simple, minimally invasive and feasible, and this method of model creation may facilitate future experimental studies.