Objective: To investigate the feasibility and effect of resection of large benign breast tumor with an ultrasoundguided Mammotome revolve device. Methods: The clinical data of 105 patients with single breast mass (>3.0 cm) undergoing surgical treatment were retrospectively analyzed. Of the patients, 68 cases underwent ultrasound-guided Mammotome procedure (observational group), and the other 37 cases underwent traditional open surgery (controlled group). The main surgical variables between the two groups were compared. Results: There was no difference between the two groups in the baseline data such as age, disease constitution and tumor size (all P>0.05), so they were comparable. The operative time, intraoperative blood loss and incidence of postoperative complications showed no significant difference between the two groups (P>0.05), but the length of the incisions in observational group was significantly less than that in control group [(0.53±0.04) cm vs. (3.65±0.46) cm, P<0.05]. Follow-up was conducted for 1 to 12 months in the two groups of patients, no tumor recurrence occurred, and scars of different lengths were noted in patients in control group, while the scars were very small or invisible in those in observational group. Conclusion: Resection of large benign breast tumor with ultrasound-guided Mammotome revolve device is safe and feasible, and offers favorable cosmetic results, so it has applicable value in clinical practice.