文章摘要

表皮生长因子受体抑制剂联合5- 氟尿嘧啶对胆管癌细胞增殖与迁移、侵袭能力的影响

作者: 1曾杰宏, 1彭思远, 1谢博文, 1刘铮凯, 1陈晨, 1李浩, 1蒋波
1 湖南师范大学第一附属医院/ 湖南省人民医院 肝胆外科，湖南 长沙 410006
通讯: 蒋波 Email: jiangbo@medmail.com.cn
DOI: 10.3978/.10.3978/j.issn.1005-6947.2015.08.009
基金: 湖南省卫生厅医药卫生科研计划资助项目, B2012-088 湖南省科学技术厅科技计划资助项目, 2014FJ3033

摘要

目的：探讨表皮生长因子受体（EGFR）抑制剂AG1478 联合5- 氟尿嘧啶（5-FU）对胆管癌细胞体外增殖、迁移和侵袭的影响。方法：体外培养人胆管癌QBC939 细胞，分别用AG1478（10 μmol/L）、5-FU（100 mg/L）以及联合两药处理24 h 后，分别采用CCK-8 法、划痕试验、Matrigel 侵袭小室法检测细胞增殖、迁移及侵袭情况。结果：AG1478 与5-FU 单独作用后，QBC939 细胞增殖、迁移和侵袭能力均明显降低（均P<0.05），而与任一单独用药比较，联合用药对细胞增殖、迁移和侵袭能力的抑制作用均明显增强（均P<0.05）。结论：EGFR 抑制剂与5-FU 联合应用可能是抑制胆管癌细胞生长、迁移和侵袭有效方法。
关键词： 胆管肿瘤 受体，表皮生长因子 氟尿嘧啶 抗肿瘤联合化疗方案

Effects of epidermal growth factor receptor inhibitor plus 5-fluorouracil on proliferation, migration and invasiveness of cholangiocarcinoma cells

Authors: 1ZENG Jiehong, 1PENG Siyuan, 1XIE Bowen, 1LIU Zhengkai, 1CHEN Chen, 1LI Hao, 1JIANG Bo
1 Department of Hepatobiliary Surgery, the First Affiliated Hospital, Hunan Normal University/Hunan Provincial Hospital, Changsha 410006, China

CorrespondingAuthor:JIANG Bo Email: jiangbo@medmail.com.cn

Abstract

Objective: To investigate the effects of epidermal growth factor receptor (EGFR) inhibitor AG1478 in combination with 5-fluorouracil (5-FU) on proliferation, migration and invasiveness of cholangiocarcinoma cells in vitro. Methods: Human cholangiocarcinoma QBC939 cells were cultured in vitro and exposed to AG1478 (10 μmol/L) and 5-FU (100 mg/L) alone or in combination for 24 h. The cell proliferation, migration and invasiveness were measured by CCK-8 assay, wound-scratch assay and Matrigel invasion chamber assay, respectively. Results: The abilities of proliferation, migration and invasion of QBC939 cells were significantly decreased after either lone AG1478 or 5-FU treatment (all P<0.05), and above effects were further enhanced by the combined treatment compared with either lone drug treatment (all P<0.05). Conclusion: Combination use of EGFR inhibitor and 5-FU may be an effective method for inhibiting the growth, migration and invasiveness of cholangiocarcinoma cells.
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