甲状腺乳头状癌与桥本甲状腺炎中上皮膜抗原的表达及意义
| 作者: |
1杨岳,
2张志勇,
1房辉,
3胡耀杰,
2吴晨鹏,
1赵青梅,
2李雪梅
1 河北省唐山市工人医院内分泌科，河北 唐山 063000 2 河北省唐山市工人医院病理科，河北 唐山 063000 3 河北省唐山市工人医院头颈外科，河北 唐山 063000 |
| 通讯: | |
| DOI: | 10.3978/.10.3978/j.issn.1005-6947.2016.05.008 |
摘要
目的：探讨上皮膜抗原（EMA）在甲状腺乳头状癌（PTC）与桥本甲状腺炎组织中的表达及临床意义。方法：用免疫组化法检测54例PTC患者癌组织，连同14例淋巴结转移癌、12例癌旁桥本甲状腺炎组织以及10例正常甲状腺组织中EMA蛋白的表达，分析其与患者临床病理特征的关系。结果：EMA蛋白表达于在胞浆及胞膜/腔缘。胞浆内EMA蛋白在正常甲状腺组织、桥本甲状腺炎组织、PTC组织、淋巴结转移癌组织中的阳性表达率差异有统计学意义（P<0.05），分别为80.0%、25.0%、64.8%、35.7%；胞浆内EMA蛋白的阳性表达与患者病灶大小有关（P<0.05）。胞膜/腔缘EMA蛋白在正常甲状腺组织、桥本甲状腺炎组织、PTC组织、淋巴结转移癌组织中的阳性表达率差异有统计学意义（P<0.05），分别为10.0%、100.0%、44.4%、64.3%；胞膜/腔缘EMA蛋白阳性表达与患者的临床病理因素均无关（均P>0.05）。结论：胞膜/腔缘EMA蛋白高表达可能提示桥本甲状腺炎更易发生PTC；胞浆内EMA蛋白表达降低可考虑作为甲状腺微小癌筛查的辅助指标之一。
关键词：
甲状腺肿瘤
癌，乳头状
桥本病
黏蛋白-1
Expression of epithelial membrane antigen in Hashimoto’s thyroiditis and papillary thyroid carcinoma and its significance
Abstract
Objective: To investigate the expression of epithelial membrane antigen (EMA) in tissue of papillary thyroid carcinoma (PTC) and Hashimoto’s thyroiditis and the clinical significance. Methods: The EMA protein expression in specimens of PTC tissue from 54 patients and tissue specimens of PTC adjacent Hashimoto's thyroiditis in 15 cases and metastatic lymph nodes in 12 cases, and normal thyroid tissues from 10 subjects were determined by immunohistochemical staining. The relations of EMA protein expression with the clinicopathologic features of the patients were analyzed. Results: EMA protein expression was found in the cytoplasm and cell membrane/inner surface of cell membrane. The positive expression rate of cytoplasm EMA in normal thyroid tissue, tissue of Hashimoto’s thyroiditis, PTC and lymph node metastases was 80.0%, 25.0%, 64.8%, and 35.7% respectively, and the difference had statistical significance (P<0.05). The cytoplasm EMA positive expression was significantly associated with the tumor size of the patients (P<0.05). The positive expression rate of EMA in the cell membrane/inner surface of cell membrane in normal thyroid tissue, tissue of Hashimoto’s thyroiditis, PTC and lymph node metastases was 10.0%, 100.0%, 44.4% and 64.3% respectively, and the difference had statistical significance (P<0.05); the EMA positive expression in the cell membrane/inner surface of cell membrane was irrelevant to any clinicopathologic factors of the patients (all P>0.05). Conclusion: High EMA expression in the cell membrane/inner surface of cell membrane may suggest a high possibility of transformation of Hashimoto’s thyroiditis to PTC. Decreased EMA expression in the cytoplasm can probably be used as an auxiliary index for screening of thyroid microcarcinoma.
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