下调NDRG1 表达对胰腺癌细胞增殖与凋亡的影响
| 作者: |
1张小薄,
1高峰,
1谭晓冬,
1周磊,
1王怀涛,
2石刚
1 中国医科大学附属盛京医院 普通外科，辽宁 沈阳110004 2 辽宁省肿瘤医院 大肠外科，辽宁 沈阳 110042 |
| 通讯: |
谭晓冬
Email: tanxd@sj-hospital.org |
| DOI: | 10.3978/.10.3978/j.issn.1005-6947.2015.03.011 |
| 基金: | 国家自然科学基金资助项目, 30973501 |
摘要
目的：探讨NDRG1 在胰腺癌细胞中的表达及其与MMP-7 的关系。 方法： 检测NDRG1 与MMP-7 蛋白与mRNA 在4 种胰腺癌细胞株（PANC-1、BXPC-3、CAPAN-2、 SW1990）表达；构建靶向NDRG1 的干扰质粒siNDRG1，转染PANC-1 细胞，检测转染后PANC-1 细 胞NDRG1 与MMP-7 蛋白与mRNA 的表达，以及增殖与调亡情况。 结果：4 种细胞株中均有NDRG1 与MMP-7 蛋白与mRNA 的表达，且两者的表达水平随着细胞分化程 度的降低而升高（均P<0.05）；PANC-1 细胞转染siNDRG1 后，NDRG1 与MMP-7 的蛋白与mRNA 表 达均明显降低、细胞增殖明显抑制、凋亡率明显升高（均P<0.05）。 结论：NDRG1 的表达与胰腺癌细胞的分化程度密切相关，且可能通过上调MMP-7 表达促进胰腺癌细 胞的生长。
关键词：
胰腺肿瘤
N-myc 下游调节基因1
细胞分化
Influence of down-regulation of NDRG1 expression on proliferation and apoptosis in pancreatic cancer cells
CorrespondingAuthor:TAN Xiaodong Email: tanxd@sj-hospital.org
Abstract
Objective: To investigate NDRG1 expression and its relation with MMP-7 in pancreatic cancer cells. Methods: The protein and mRNA expressions of NDRG1 and MMP-7 in 4 different pancreatic cancer cell lines (PANC-1, BXPC-3, CAPAN-2 and SW1990) were determined. The interference plasmids siNDRG1 were transfected into PANC-1 cells after their construction, and then the protein and mRNA expressions of NDRG1 and MMP-7, cell proliferation and apoptosis after transfection were determined. Results: All the 4 cell lines presented either the protein or mRNA expressions of both NDRG1 and MMP-7, and their expression levels were increased with decrease of the degree of cell differentiation (all P<0.05). In PANC- 1 after siNDRG1 trasfection, the protein and mRNA expressions of both NDRG1 and MMP-7 were reduced, proliferation was suppressed, and apoptosis rate was increased significantly (all P<0.05). Conclusion: NDRG1 expression is closely related to the degree of differentiation of pancreatic cancer cells, and may probably promote cell growth through up-regulating MMP-7 expression.
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