文章摘要

MMP-2 和MMP-9 在肝内胆管细胞癌组织中的表达及意义

作者: 1徐靖, 2易炜, 3万百顺, 4尹碧辉
1 河南省肿瘤医院放疗科，河南 郑州 450008
2 广州医科大学附属第一医院 放疗科，广东 广州510230
3 河南省肿瘤医院肝胆外科，河南 郑州 450008
4 湖北省仙桃市第一人民医院 普通外科，湖北 仙桃 433000
通讯: 易炜 Email: wanmed@126.com
DOI: 10.3978/.10.3978/j.issn.1005-6947.2015.08.010

摘要

目的：探讨基质金属蛋白酶2（MMP-2）与基质金属蛋白酶9（MMP-9）在肝内胆管细胞癌（ICC）组织中的表达及其与ICC 患者临床病理特征及预后的关系。方法：收集2011 年1 月—2014 年12 月收治的50 例ICC 患者的癌组织与癌旁组织手术标本，采用免疫组织化学方法及RT-PCR 方法检测MMP-2 和MMP-9 在以上组织中的表达，分析MMP-2 和MMP-9表达与ICC 患者临床病理参数以及术后生存率的关系。结果： 免疫组织化学结果显示，ICC 组织中MMP-2 和MMP-9 蛋白阳性表达率分别为34.0% 和32.0%，而两者在癌旁组织中无阳性表达；RT-PCR 结果显示，ICC 组织中MMP-2 和MMP-9 的相对表达均明显高于癌旁组织（均P<0.05）。MMP-2 和MMP-9 表达与ICC 患者是否存在淋巴结转移和肿瘤分化程度有关（均P<0.05），而与患者性别、年龄和肿瘤分期无关（均P>0.05）；MMP-2 和MMP-9阳性患者生存率明显低于各自阴性者，而两者均阳性患者的生存率最低（均P<0.05）。结论：ICC 组织中MMP-2 和MMP-9 表达明显上调，且两者的表达与ICC 患者恶性临床病理特征及不良预后密切相关。
关键词： 胆管肿瘤；胆管，肝内；基质金属蛋白酶2；基质金属蛋白酶9；预后

MMP-2 and MMP-9 expressions in intrahepatic cholangiocarcinoma tissue and the significance

Authors: 1XU Jing, 2YI Wei, 3WAN Baishun, 4YIN Bihui
1 Department of Radiotherapy, Henan Cancer Hospital, Zhengzhou 450008, China
2 Department of Radiotherapy, the First Affiliated Hospital, Guangzhou Medical University, Guangzhou 510230, China
3 Department of Hepatobiliary Surgery, Henan Cancer Hospital, Zhengzhou 450008, China
4 Department of General Surgery, Xiantao First People’s Hospital, Xiantao, Hubei 433000, China

CorrespondingAuthor:YI Wei Email: wanmed@126.com

Abstract

Objective: To investigate the expression of matrix metalloproteinase 2 (MMP-2) and 9 (MMP-9) in intrahepatic cholangiocarcinoma (ICC) and their relations with the clinicopathologic features and prognosis of ICC patients. Methods: The surgical specimens including tumor and tumor-adjacent tissue from 50 ICC patients treated between January 2011 and December 2014 were collected. The MMP-2 and MMP-9 expressions in these tissues were detected by immunohistochemical staining and RT-PCR method, and the relations of MMP-2 and MMP-9 expressions with clinicopathologic variables and postoperative survival of ICC patients were analyzed. Results: Results of immunohistochemical staining showed that the positive expression rate of MMP-2 and MMP- 9 in ICC tissue was 34.0% and 32.0% respectively, but neither was positive in tumor-adjacent tissue; results of RTPCR showed that both relative expression levels of MMP-2 and MMP-9 in ICC tissue were significantly higher than those in tumor-adjacent tissue (both P<0.05). Both MMP-2 and MMP-9 expressions were significantly associated with the presence or absence of lymph node metastasis and degree of tumor differentiation of ICC patients (all P<0.05), but were irrelevant to the gender, age and tumor stage of ICC patients (all P>0.05); the survival rate in patients with positive MMP-2 or MMP-9 expression was significantly lower than that in patients with negative MMP-2 or MMP-9 expression, and those with both MMP-2 and MMP-9 positive expressions had lowest survival rate (all P<0.05). Conclusion: MMP-2 and MMP-9 expressions are increased in ICC tissue, and both expressions are closely related to the malignant clinicopathologic profiles and poor prognosis of ICC patients.
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