HSF-1在HMGB1诱导的炎症反应中的作用
作者: |
1尹朝奇,
1贺全勇,
1罗成群,
1周建大,
1陈佳,
1李萍,
1朱颉,
1彭浩,
1徐阳成,
1陶如意
1 中南大学湘雅三医院 烧伤整形科,湖南 长沙 410013 |
通讯: |
尹朝奇
Email: yinchaoqi@163.com |
DOI: | 10.3978/.10.3978/j.issn.1005-6947.2016.09.011 |
基金: | 湖南省卫生厅科研基金资助项目, B2012-030 |
摘要
目的:探讨热休克转录因子1(HSF-1)在高迁移率族蛋白1(HMGB1)所诱导的炎症反应中的作用。方法:不同浓度HMGB1作用于RAW264.7细胞不同时间后,用ELISA法检测细胞上清液TNF-α水平;分别用免疫荧光法与Western blot法检测HMGB1作用后,RAW264.7细胞NF-κB核转移情况与HSF-1表达。观察干扰HSF-1表达后,HMGB1诱导RAW264.7细胞TNF-α表达的变化。结果:HMGB1作用后,RAW264.7细胞分别在4、12 h出现两次TNF-α分泌高峰,且TNF-α的释放量随HMGB1浓度的增加而增加。HMGB1作用后,RAW264.7细胞的NF-κB核转移明显增强,HSF-1表达明显增加(均P<0.05)。干扰HSF-1表达后,HMGB1诱导RAW264.7细胞产生的TNF-α量较未干扰的RAW264.7细胞明显增加(P<0.05)。结论:HMGB1能诱导RAW264.7细胞的炎症反应与HSF-1的表达,但HSF-1可能对HMGB1诱导的炎症反应有负反馈性抑制作用。
关键词:
胰腺炎,急性坏死性
全身炎症反应综合征
高迁移率族蛋白质类
热休克转录因子1
Role of HSF-1 in inflammatory response induced by HMGB1
CorrespondingAuthor:YIN Chaoqi Email: yinchaoqi@163.com
Abstract
Objective: To investigate the role of heat shock transcription factor 1 (HSF1) in inflammatory response induced by high mobility group protein B1 (HMGB1). Methods: The TNF-α contents in the supernatants of RAW264.7 cells after exposure to different concentrations of HMGB1 for different times were determined by ELISA assay. The NF-κB nuclear translocation and HSF-1 expression in RAW264.7 cells after HMGB1 treatment were examined by immunofluorescence and Western blot, respectively. The change in TNF-α expression induced by HMGB1 in RAW264.7 cells after interference of HSF-1 expression was observed. Results: RAW264.7 cells presented two peaks of TNF-α release at 4 and 12 h respectively after HMGB1 treatment, and the TNF-α release level was increased with the elevation of HMGB1 concentration. The NF-κB nuclear translocation was significantly enhanced and HSF-1 expression was significantly increased in RAW264.7 cells after HMGB1 treatment (both P<0.05). The TNF-α expression induced by HMGB1 in RAW264.7 cells after interference of HSF-1 expression was significantly increased compared with RAW264.7 cells without HSF-1 interference (P<0.05). Conclusion: HMGB1 can induce inflammatory response and HSF-1 expression in RAW264.7 cells, but HSF-1 may play a negative feedback inhibitory role in the HMGB1-induced inflammation.
Keywords: