Objective: To investigate the efficacy of staged sequential minimally invasive approach for acute biliary pancreatitis (ABP). Methods: From January 2013 to December 2015, 112 patients with ABP received basic conservative treatment together with staged sequential minimally invasive management, which included endoscopic nasobiliary drainage (ENBD) followed by endoscopic sphincterotomy (EST) plus basket stone extraction, and then, elective laparoscopic cholecystectomy (LC) or LC plus laparoscopic common bile duct exploration (LCBDE), and individualized treatment based on minimally invasive therapy. Results: In all 88 patients with mild or moderate disease, the etiologic biliary stones were extracted during hospitalization, and they were cured and discharged from hospital with no mortality and an average length of hospital stay of (7.5±2.3) d; in the 24 patients with severe disease, 2 cases died of multi-organ failure, and the remaining cases were cured and discharged from hospital uneventfully, with an average length of hospital stay of (21.2±5.3) d. Conclusion: Staged sequential minimally invasive method has definite efficacy in treatment of ABP, with advantages of thorough resolution of the cause of disease during one-admission and reduced length of hospitalization.