Objective: To evaluate the efficacy and safety of using 3D laparoscopic technique in gastric carcinoma surgery. Methods: The randomized clinical trials regarding using 3D laparoscopic technique in gastric carcinoma surgery were collected by searching several national and international online databases. Article screening, data extraction and quality assessment were performed by two independent reviewers according to the defined inclusion and exclusion criteria, and then, Meta-analysis was performed by using RevMan 5.2 software. Results: Seven studies were finally included with 650 patients. Results of Meta-analysis showed that the operative time was shortened (MD=–0.59, 95% CI=–0.76––0.42, P<0.000 01), and intraoperative blood loss was reduced (MD=–0.56, 95% CI=–0.73--0.39, P<0.000 01), while hospitalization expense was increased (MD=378.42, 95% CI=171.81–585.04, P=0.000 3) in 3D laparoscopic surgery compared with control group (2D laparoscopic surgery). Results of the included studies indicated that other efficacy parameters and safety of 3D laparoscopic surgery had no significant difference compared with control group (all P>0.05). Conclusion: 3D laparoscopic assisted surgery for gastric carcinoma has advantage in terms of operative duration and blood loss, and it also has better surgical safety, but the hospitalization cost may increase.